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Home » Products » Elisa Kits » 3-NT(3-Nitrotyrosine) ELISA Kit

3-NT(3-Nitrotyrosine) ELISA Kit

3-NT(3-Nitrotyrosine) ELISA Kit
3-NT(3-Nitrotyrosine) ELISA Kit
Product Code : E-EL-0040
Brand Name : 3-NT(3-Nitrotyrosine
Product Description

Intended use

This ELISA kit can be applied to the in vitro quantitative determination of  3-NT concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 3-NT. During the reaction,  3-NT in the sample or standard competes with a fixed amount of 3-NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  3-NT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay type Competitive
Format 96T
Assay time 2.5h
Reactivity Universal
Detection Method Colormetric
Detection Range 1.56—100 ng/mL
Sensitivity 0.94 ng/mL
Sample Volume 50μL
Sample Type Serum, plasma and other biological fluids

Specificity

This kit recognizes 3-NT in samples. No significant cross-reactivity or interference was observed.

 

Assay Procedure

1.Add 50 μL of standard or sample to each well.

2.Immediately add 50 μL of Biotinylated Detection Ab to each well.

3.Incubate for 45 min at 37℃. Aspirate and wash 3 times.

4.Add 100 μL of HRP Conjugate to each well. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

5.Add 90 μL of Substrate Reagent. Incubate for 15 min at 37℃.

6.Add 50 μL of Stop Solution.

7.Read the OD at 450 nm immediately. Calculation of the results.


SR GROUP

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