A-549 Cell line
Product Details:
- Other Names A549, Human Lung Carcinoma Epithelial Cells
- Smell Odorless
- Appearance Adherent monolayer cells
- Storage Liquid nitrogen or -80C for cryopreserved vial
- Poisonous Non-poisonous (biological hazardhandle as per biosafety guidelines)
- HS Code 30029030
- Usage For research use only, not for diagnostic or therapeutic use
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A-549 Cell line Price And Quantity
A-549 Cell line Product Specifications
- White to off-white (as cell pellet)
- Adherent monolayer cells
- Odorless
- A549, Human Lung Carcinoma Epithelial Cells
- Liquid nitrogen or -80C for cryopreserved vial
- > 95% Viability
- Non-poisonous (biological hazardhandle as per biosafety guidelines)
- 30029030
- Cryopreserved vial or culture flask
- For research use only, not for diagnostic or therapeutic use
- Research Grade
- Polygonal, Epithelial-like
- A-549 Cell Line
- 7.2-7.4
- Cancer research, cytotoxicity assays, drug screening, virology, and gene expression studies
- Cell Culture
- 1 year (in liquid nitrogen)
- Human Adenocarcinoma Epithelial Cell Line
A-549 Cell line Trade Information
- Cash in Advance (CID)
- All India
Product Description
A-549 Cell line
Cells are of various human (A549 lung carcinoma, HCT116 colon carcinoma, HEK293 human embryonic kidney cells, Hela cervical carcinoma, Jurkat T lymphocytes, Ramos B lymphocytes, THP-1 monocytes) or murine (B16 melanoma, J774 and RAW 264.7 macrophages) origins. Our cell lines are stably transfected with reporter constructs encoding the secreted embryonic alkaline phosphatase (SEAP) secreted Lucia luciferase gene downstream of a response element that is specific for the signaling pathway being studied.cell lines are available for double readout with both SEAP and Lucia luciferase
Key Features of A-549 Cell Line
A-549 cells are human lung adenocarcinoma epithelial cells displayed in an adherent monolayer, with a polygonal morphology and purity greater than 95% viability. They are mycoplasma-free, authenticated via DNA profiling, and supplied in a cryopreserved vial or culture flask. Their doubling time is approximately 2224 hours, making them reliable for consistent experimental outcomes.
Medium and Culturing Requirements
For optimal growth, A-549 cells require DMEM medium supplemented with 10% Fetal Bovine Serum (FBS). Recommended subculture ratios range from 1:3 to 1:8. Antibiotics are not included unless specifically requested. Maintenance at a pH level of 7.27.4 secures ideal cell health and performance during research.
Applications and Advantages
A-549 Cell Line is widely used in cancer research, cytotoxicity studies, drug screening, virology, and gene expression analysis. Its high purity and rapid growth facilitate reproducible experimental results, making it a preferred choice among researchers seeking robust, validated human lung carcinoma models for preclinical investigation.
FAQs of A-549 Cell line:
Q: How should A-549 Cell Line be stored after receiving the cryopreserved vial?
A: Upon receipt, A-549 cryopreserved vials should be stored in liquid nitrogen or at -80C to preserve cell viability and ensure shelf life of one year. Follow standard thawing procedures when establishing cultures.Q: What is the recommended medium for culturing A-549 cells?
A: A-549 cells perform best in DMEM medium supplemented with 10% Fetal Bovine Serum (FBS). Adjust the pH to 7.27.4 to optimize cell growth and survival.Q: When should subculturing be performed for A-549 cells?
A: Subculturing is recommended when cells reach 7080% confluency. Use a ratio of 1:3 to 1:8 for passaging to maintain healthy cultures and consistent experimental outcomes.Q: Where can the A-549 cell line be utilized in research?
A: A-549 cells find application in cancer research, cytotoxicity and drug screening assays, virology, and gene expression studies. Their human lung carcinoma origin makes them suitable for respiratory and oncology-focused investigations.Q: What process is used to confirm the identity and purity of the A-549 cell line?
A: Authentication of A-549 cell line is performed through DNA profiling and STR analysis, ensuring genetic integrity, while regular mycoplasma testing verifies contamination-free cultures (>95% viability is guaranteed).Q: How does using A-549 cells benefit scientific research?
A: Researchers benefit from A-549 cell lines rapid doubling time, high viability, and confirmed purity. These cells provide robust, reproducible results in experiments focused on human lung cancer and related biological processes.Q: Is the use of antibiotics recommended in A-549 cell culture?
A: Antibiotics are not included in standard A-549 cell cultures unless specifically required by experimental protocols. Maintaining strict aseptic technique is suggested to avoid contamination.Tell us about your requirement
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Contact Details
- Plot No- D17/103, Rohini Sector 3, Near Ram Lila Park, Delhi - 110085, India
- Phone :08045816517
- Send Inquiry
Send Inquiry- Mr Rambhajan Dwivedi (Proprietor)
- Mobile :08045816517
- Customer Care Number---011-27511805
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