Cookie Img Back to top
   Call Me Free Offline Send SMS Send Inquiry
Home » Products » Elisa Kits » AMOZ (Furaltadone Metabolite) ELISA Kit

AMOZ (Furaltadone Metabolite) ELISA Kit

AMOZ (Furaltadone Metabolite) ELISA Kit
AMOZ (Furaltadone Metabolite) ELISA Kit
Product Code : E-FS-E002
Brand Name : AMOZ (Furaltadone Me
Product Description

Test principle

This kit uses Indirect-Competitive-ELISA as the method. It can detect AMOZ in samples, such as honey, fish, shrimp, birds, live, etc. This kit is composed of Micro ELISA Plate, HRP conjugation, antibody, standard and other supplementary reagents. The micro-plate provided in this kit has been pre-coated with AMOZ. During the reaction, AMOZ in the samples or standard competes with AMOZ on the solid phase supporter for sites of AMOZ antibody. Then Horseradish Peroxidase (HRP) conjugation is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of AMOZ. The concentration of AMOZ in the samples can be calculated by comparing the OD of the samples to the standard curve.

Technical indicator

Sensitivity: 0.05 ppb (ng/mL)

Reaction mode: 25℃, 45 min~15 min

Detection limit: Tissue/liver---0.1 ppb, Honey/milk/casing---0.1 ppb, 

                             Milk powder/egg powder/fodder---0.1 ppb, Fish and shrimp: 0.15 ppb

Cross-reactivity: Furaltadone metabolite---100%, Furazolidone metabolite---0.1%,

                               Nitrofurantoin metabolite---0.1%, Nitrofurazone metabolite---0.1%

Sample recovery rate: Tissue/liver ---80%±25%,  Honey/milk/casing---75%±15%, 

                                          Milk powder/egg powder/fodder---85%±25%

Kits components

Item

Specifications

Micro ELISA Plate

96 wells

Standard Liquid

1 mL each

(0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb, 4.05 ppb)

High Concentrated Standard (100 ppb)

1 mL

Derivatization Reagent (black cap)

10 mL

HRP Conjugate (red cap)

5.5 mL

Antibody Working Solution (blue cap)

5.5 mL

Substrate Reagent A (white cap)

6 mL

Substrate Reagent B (black cap)

6 mL

Stop Solution (yellow cap)

6 mL

20×Concentrated Wash Buffer (white cap)

40 mL

2×Reconstitution Buffer (yellow cap)

50 mL

Plate Sealer

3 pieces

Sealed Bag

1 piece

Manual

1 copy

 

Other supplies required

Instruments: Micro-plate reader, Printer, Homogenizer, Nitrogen Evaporators, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

High-precision transferpettor: Single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).

Reagents: Ethyl acetate, n-hexane, sodium hydroxide, concentrated HCl, K2HPO4•3H2O, K2Fe (CN) 5(NO) •2H2O, ZnSO4•7H2O.

Storage and valid period

Storage: Store at 2-8℃. Avoid freeze / thaw cycles.

Valid Period: 1 year, production date is on the packing box.




SR GROUP

Contact Us

( *represents compulsory fields )

Tips on getting accurate quotes. Please include product name, order quantity, usage, special requests if any in your inquiry.