Product Description
Anti-HA (HA.C5)Tag ( Monoclonal Antibody 100UG
EBI's Anti-HA Tag (HA.C5) mAb, similar to our Anti-His Tag (HIS.H8) mAb, has also enjoyed strong praise from our customers, du to its high affinity and high specificity.
To Order
| Catalog No. | T002 | E-mail | , , |
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| Unit Size Pack and Price | |
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| | pack size | price | | 50 ug Lyophilize | 8,000/- | | 100 ug Lyophil | 13,000/- | | 50 ug liquid | 9,000/- | | 100 ug liquid | 14000/- |
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| | Mobile (India ) | 9990521622 |
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Data Sheet
| Print Version | Anti-HA Tag PDF Data Sheet (requires PDF Reader) |
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| Description | Anti-HA Tag (HA.C5) Monoclonal Antibody |
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| Clonality | HA.C5 |
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| Host Species | Mouse |
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| Isotype | IgG3 |
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| Immunogen | YPYDVPDYA (HA) synthetic peptide conjugated to KLH. |
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| Specificity | Recognizes the N-terminal, C-terminal or internal HA-tagged fusion proteins. |
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| Purification | Protein A affinity chromatography from mouse ascites fluid. |
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| Applications | Dot, ELISA, IP, IS, WB (see notations). |
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| Sensitivity | WB (with ECL): 1-5ng of HA-tagged fusion proteins. |
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| Form | Liquid: 1mg/mL in 10mM PBS, pH 7.2, 0.05% NaN3. Lyophilized: 1mg/mL lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% NaN3. |
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| Working Conditions | WB (with ECL): 1:1,000-3,000 dilution (incubate for one hour at room temperature). IS: 1:500-2000 dilution. For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test. |
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| Storage | Liquid: Store at -20C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4C for several days to weeks. Lyophilized: Store at -20C for 3 years or more. Reconstitute with distilled water to desired concentration before use. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4C for several days to weeks. See Storage for more information. |
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| Images | Fig. 1:1,000 (1g/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (1) and transfected (2). |
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For in vitro research use only
High Specificity for HA-tagged ProteinsAnti-HA Tag (HA.C5) Monoclonal Antibody ensures precise recognition of HA-tagged recombinant proteins, thanks to its highly specific binding to the YPYDVPDYA epitope. Its excellent purity and validated performance make it effective for multiple research applications, from Western blotting to immunofluorescence and ELISA.
Versatile Applications in Molecular BiologyThis monoclonal antibody can be used in a range of assays, including Western blot (1:1,0001:5,000), immunoprecipitation, immunofluorescence/ICC (1:1001:500), and ELISA (1:2,0001:10,000). Researchers can confidently detect and analyze HA-tagged proteins in various sample types, enhancing the reliability of experimental outcomes.
Convenient Sizes and Reliable StorageAvailable in both 100 g and 50 g vials, the Anti-HA Tag (HA.C5) Antibody is competitively priced for research labs (13,000 and 7,000, respectively). The formulation remains stable when stored at -20C or below, with a shelf life of 12 months, making it easy to integrate into routine laboratory workflows.
FAQs of Anti-HA Tag (HA.C5) Monoclonal Antibody price @ 13000 only for 100 ug and price @7000 for 50ug:
Q: How should the Anti-HA Tag (HA.C5) Monoclonal Antibody be stored for optimal stability?
A: For best results, store the antibody at -20C or below. Avoid multiple freeze-thaw cycles to maintain its integrity. Under proper storage, it remains stable for 12 months from the date of manufacture.
Q: What are the recommended working dilutions for different applications?
A: The antibody can be diluted 1:1,0001:5,000 for Western blot, 1:1001:500 for immunofluorescence/ICC, and 1:2,0001:10,000 for ELISA. Optimal dilution may vary based on specific experimental conditions.
Q: Where can I use the Anti-HA Tag (HA.C5) Monoclonal Antibody, and what is its main benefit?
A: This antibody is suitable for a variety of assays, including Western blot, immunoprecipitation, immunofluorescence, and ELISA, offering accurate detection of HA-tagged proteins and facilitating streamlined protein analysis in research laboratories.
Q: What is the process for ordering the antibody, and are additional documents like a Certificate of Analysis provided?
A: You can order the antibody from authorized distributors, importers, or suppliers in India. A Certificate of Analysis is available upon request to support quality assurance in your research.
Q: When will my order be shipped and what are the shipping conditions?
A: Orders are shipped on ice packs to preserve the antibodys quality during transportation, ensuring it reaches your lab in optimal condition regardless of location.
Q: How does this monoclonal antibody differ from polyclonal antibodies for HA-tag detection?
A: Unlike polyclonal antibodies, this monoclonal antibody offers consistency, high specificity, and reduced background by targeting a single epitope (HA tag), making it ideal for reproducible and reliable experimental results.