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Home » Products » Elisa Kits » Bulk Request Rat GM-CSF(Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit

Bulk Request Rat GM-CSF(Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit

Bulk Request Rat GM-CSF(Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit
Bulk Request Rat GM-CSF(Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit
Product Code : :E-EL-R0008
Brand Name : Rat GM-CSF
Product Description

Rat GM-CSF(Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit Synonyms:GMCSF, CSF2

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat GM-CSF concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GM-CSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GM-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GM-CSF. You can calculate the concentration of Rat GM-CSF in the samples by comparing the OD of the samples to the standard curve.

Assay typeSandwich
Format96T
Assay time4.5h
ReactivityRat
Detection MethodColormetric
Detection Range15.63—1000 pg/mL
Sensitivity9.38 pg/mL
Sample Volume Required Per Well100 μL
Sample TypeSerum, plasma and other biological fluids

Specificity

This kit recognizes Rat GM-CSF in samples. No significant cross-reactivity or interference between Rat GM-CSF and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)O.DAverageCorrected
10002.399
2.405
2.4022.342
5001.641
1.661
1.6511.591
2500.917
0.877
0.8970.837
1250.472
0.494
0.4830.423
62.50.243
0.221
0.2320.172
31.250.171
0.149
0.160.1
15.630.105
0.119
0.1120.052
00.05
0.07000000000000001
0.06--

 

Kit components & Storage

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells ×12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL4℃, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL4℃(shading light)
Stop Solution1 vial, 10 mL4℃
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy

Assay Procedure

1.Add 100 μL of standard or sample to each well.

2.Remove the liquid.

3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃.Aspirate and wash 3 times.

4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃.Aspirate and wash 5 times.

5.Add 90 μL of Substrate Reagent. Incubate for 15 min at 37℃.

6.Add 50 μL of Stop Solution.

7.Read the OD at 450 nm immediately. Calculation of the results.


SR GROUP

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