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Home » Products » Elisa Kits » cGMP ELISA Kit 96 test

cGMP ELISA Kit 96 test

cGMP ELISA Kit 96 test
cGMP ELISA Kit 96 test
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Product Code : E-EL-DS02
Brand Name : cGMP ELISA Kit
Price And Quantity
  • Price
  • 28000 INR
Product Description

Introduction

Guanosine 3’, 5’-cyclic monophosphate (cGMP) is a key second messenger molecule in intracellular signal transduction. Monitoring cGMP levels is one of the most common ways to screen for agonists and antagonists of G protein–coupled receptors (GPCRs).

Assay type     

Competitive

Format

96T

Detection method

Colorimetric

IC50

5 pmol/mL

Detection limit

0.1 pmol/mL

Elabscience® cGMP ELISA Kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with goat anti-mouse IgG. During the reaction, sample or standard, cGMP-HRP Conjugate and mouse monoclonal anti-cGMP antibody (cGMP MAb) are sequentially added to each wells. cGMP in the sample or standard competes with a fixed amount of HRP-labeled cGMP for the binding sites of cGMP MAb. Free components are washed from the plate. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of cGMP in the samples is then determined by comparing the OD of the samples to the standard curve. The ELISA typically displays an IC50 (50% B/B0) of approximately 5 pmol/mL and a detection limit of approximately 0.1 pmol/mL.

Kit components

Item

Specification

Storage

Goat anti-mouse IgG Micro-plate (12 × 8 wells)

96 wells

4℃

cGMP Standard (10000 pmol/mL)

0.1 mL

-80℃

cGMP-HRP Conjugate (1000×)

0.02 mL

-80℃

Anti-cGMP McAb (1000×)

0.02 mL

-80℃

Assay Buffer (10×)

30 mL

4℃

Neutralizing Buffer

8 mL

4℃

Sample Diluent

30 mL

4℃

TMB Substrate

18 mL

4℃

Stop Solution

8 mL

4℃



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