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Home » Products » Elisa Kits » DA(Dopamine) ELISA Kit

DA(Dopamine) ELISA Kit

DA(Dopamine) ELISA Kit
DA(Dopamine) ELISA Kit
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Product Code : E-EL-0046
Brand Name : Dopamine
Product Description

Intended use

This ELISA kit can be applied to the in vitro quantitative determination of  DA concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with DA. During the reaction,  DA in the sample or standard competes with a fixed amount of DA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  DA. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  DA in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay type Competitive
Format 96T
Assay time 2.5h
Reactivity Universal
Detection Method Colormetric
Detection Range 31.25—2000 pg/mL
Sensitivity 18.75 pg/mL
Sample Volume 50μL
Sample Type Serum, plasma and other biological fluids

Specificity

This kit recognizes DA in samples. No significant cross-reactivity or interference was observed.








































Recovery

The recovery of  DA  spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Range (%) Average Recovery (%)
Serum (n=5) 95-110 101
EDTA plasma (n=5) 97-111 102
Cell culture media (n=5) 92-103 98

Linearity

Samples were spiked with high concentrations of Human ACTH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 92-106 95-109 91-103
Average (%) 99 103 98
1:4 Range (%) 91-103 89-105 105
Average (%) 96 96 105
1:8 Range (%) 91-103 92-104 99-113
Average (%) 96 99 105
1:16 Range (%) 86-98 91-105 93-108
Average (%) 92 99 100

Kit components & Storage

The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.

Item Specifications Storage
Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
Reference Standard 2 vials
Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 4℃(shading light)
Stop Solution 1 vial, 10 mL 4℃
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.


 


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