detection and presumptive identification of pathogenic microorganisms from foodstuffs

Product Description

FOOD SYSTEM System for detection and presumptive identification of pathogenic microorganisms from foodstuffs validated to ISO 16140 standard

KIT CONTENTS

The kit contains: 20 FOOD SYSTEM (40 tests) 1 TEST RESULT FORM 40 vials of Physiological solution (4.5 mL/vial) 20 Sealing films 1 Instruction sheet ITEMS NECESSARY BUT NOT INCLUDED IN THE KIT VASELIN OIL (ref. 80278) H2O2 Reagent (ref. 80057) OXIDASE TEST STICK (ref. 88029) BUFFERED PEPTONE WATER (ref. 24099, 412090, 611014) KOVAC’S – REAGENT (ref. 80270) RINGER’S Solution (ref. 81059) Sundry materials used in microbiology laboratories; cover slides and slides; microscope.

PRINCIPLE OF THE METHOD

FOOD SYSTEM allows the detection and presumptive identification of the following microorganisms: Salmonella spp., Proteus spp. / Providencia spp., Pseudomonas spp., Staphylococcus aureus, E. coli, E. coli 0157, Bacillus cereus, Listeria spp., yeasts and moulds. • The presence of Salmonella spp. is shown by a yellow to red-orange colour change of well 1-LDC and by the yellow to black color change of well 2-H2S. The confirmation of Salmonella spp. is performed by taking a drop of the culture broth from the well 2-H2S and plating it onto a selective agar medium (i.e. XLD agar, ref. 10056). Proceed to identification of bacterial colonies according to ISO 6579:2004 2. The yellow to black color change of well 2-H2S and no color change in the well 1-LDC can indicate the presence of Citrobacter spp. • The presence of Proteus spp. / Providencia spp. is shown by the yellow to red-fuchsia color change of the well 3-UR, and by the yellow to brown-black color change of the well 4-PRO. • The presence of Pseudomonas spp. is shown by the yellow to turbid green colour change of the well 5-PSE. The confirmation of Pseudomonas spp. is performed by the oxidase test directly in the well (OXIDASE TEST STICK ref. 88029). • The presence of Staphylococcus aureus is shown by the appearance of a black ring on the bottom of the well 6-STA. The confirmation of Staphylococcus aureus is performed by taking a drop of the culture broth from the well 6-STA by means of a sterile loop, and plating it onto a selective agar medium (i.e. Baird Parker Agar + RPF, ref. 10521). Proceed to identification of bacterial colonies according to ISO 6888-2:1999 3. • The presence of E. coli and E. coli 0157 is shown by the red to blue color change of the well 7-ESC and by the formation of a pink-red coloring following the addition of Kovac’s reagent into the well 8-IND. • The presence of Bacillus cereus is shown by the yellow to turbid green color change of the well 9-BCE. The confirmation of Bacillus cereus is performed by taking a drop of the culture broth from the well 9-BCE by means of a sterile loop, and plating it onto a selective agar medium (i.e. Bacillus Cereus Agar-MYP, ref. 10027). Proceed to identification of bacterial colonies according to ISO 7932:2004 4. • The presence of Listeria spp. is shown by the yellow to black color change of the well 10-LIS and by the development of bubbles following the addition of Reagent H2O2 into the well 11-CAT. The confirmation of Listeria spp. is performed by taking a drop of the culture broth from the well 10-LIS by means of a sterile loop, and plating it onto a selective agar medium (i.e. O.A. Listeria Agar, ref. 10620). Proceed to identification of bacterial colonies according to ISO 11290:2002 5. • The presence of yeasts and moulds is shown by the green to yellow color change of the well 12-Y/M and by