Product Description
Based on immunoelectrophoresis, the antibody reacts with whole molecule guinea pig IgG. It also reacts with the light chains of other guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
Physical State: Freeze-dried solid Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Versatile Research ApplicationsThis antibody is well-suited for a range of immunoassays including ELISA, Western Blot, immunohistochemistry, and immunofluorescence. Its high specificity and purity make it ideal for reliably detecting Guinea Pig IgG in complex samples, providing accurate results for laboratory research.
Quality and StabilityFormulated in sterile, phosphate buffered saline and often enhanced with protein stabilizers like BSA, this reagent ensures consistent performance. Offered either as a colorless to pale yellow liquid or as a lyophilized powder, it is easy to handle and adaptable to your preferred storage conditions.
Flexible Conjugation OptionsGoat Anti-Guinea Pig IgG (H+L) may be obtained unconjugated or conjugated with HRP, FITC, or biotin. This flexibility enables its seamless integration into diverse assay formats, streamlining experimental workflows and supporting multiple detection modalities in your research.
FAQs of Goat Anti-Guinea Pig IgG (H+L):
Q: How should Goat Anti-Guinea Pig IgG (H+L) be stored for optimal stability?
A: For long-term preservation, store the antibody at or below -20C. For short-term use, refrigeration between 28C is sufficient. Protect from repeated freeze-thaw cycles to maintain maximum efficacy and shelf life.
Q: What applications is this antibody suitable for?
A: This polyclonal secondary antibody is designed for use in ELISA, Western Blot, immunohistochemistry, immunofluorescence, and various other immunoassays involving Guinea Pig IgG detection.
Q: When should I use conjugated versus unconjugated formats?
A: Choose a conjugated form (e.g., HRP, FITC, or biotin) when you need a built-in detection system matched to your assays requirements. Opt for the unconjugated version if you prefer to use customized detection reagents or require additional conjugation flexibility.
Q: Where can I find product specifications for purity and formulation?
A: All essential specifications, including minimum purity (95% as per SDS-PAGE), buffer composition, preservative content, and physical form, are outlined in the product documentation and technical data sheets supplied by the provider.
Q: What is the recommended dilution for laboratory assays?
A: Dilution recommendations vary by application; however, typical working ranges are 1:1,000 to 1:10,000. It is advisable to optimize dilution based on the specific protocol and sample type used in your experiments.
Q: How does this antibody benefit my laboratory research?
A: With its high specificity, robustness, and versatility in multiple assay formats, Goat Anti-Guinea Pig IgG (H+L) streamlines detection of Guinea Pig IgG, thereby improving experimental reliability and facilitating high-quality analytical results.