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Home » Products » Elisa Kits » Human ANG1 (Angiopoietin 1) ELISA Kit

Human ANG1 (Angiopoietin 1) ELISA Kit

Human ANG1 (Angiopoietin 1) ELISA Kit
Human ANG1 (Angiopoietin 1) ELISA Kit
Product Code : E-EL-H0007
Brand Name : ELABSCIENCE BIOTECHN
Product Description
Human ANG1 (Angiopoietin 1) ELISA Kit   , PRICE@ 465US$
Synonyms:ANG-1; ANGPT1; AGP1; AGPT

Principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and then incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANG1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ANG1 . You can calculate the concentration of Human ANG1 in the samples by comparing the OD of the samples to the standard curve.

Product ID E-EL-H0007
Assay type Sandwich ELISA
Format 96T
Assay time 4.5h
Reactivity Human
Detection Method Colormetric
Detection Range 62.500—4000.000pg/mL
Sensitivity 37.500pg/mL
Sample Volume Required Per Well 100μL
Sample Type serum, plasma and other biological fluids

Components

Item Specifications Storage
Micro ELISA Plate 8 wells ×12 strips 4℃/-20℃#
Reference Standard 2 vials 4℃/-20℃#
Reference Standard & Sample Diluent 1vial 20mL 4℃
Concentrated Biotinylated Detection Ab 1vial 120μL 4℃/-20℃#
Biotinylated Detection Ab Diluent 1vial 10mL 4℃
Concentrated HRP Conjugate 1vial 120μL 4℃(shading light)
HRP Conjugate Diluent 1vial 10mL 4℃
Concentrated Wash Buffer (25×) 1vial 30mL 4℃
Substrate Reagent 1vial 10mL 4℃(shading light)
 Stop Solution 1vial 10mL 4℃
Plate Sealer 5pieces
Manual 1 copy
Certificate of Analysis 1 copy
#: keep the kit at 4℃ if it’s used within 30 days, keep at -20℃ for longer storage.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Human ANG1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Human ANG1 were tested on 3 different plates, 20 replicates in each plate.


Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean
(pg/mL)
216.000 498.900 1629.900 226.500 533.800 1641.800
Standard deviation 14.300 20.000 60.300 15.400 27.200 50.900
C V (%) 6.600 4.000 3.700 6.800 5.100 3.100



























SR GROUP