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            Home » Products » Elisa Kits » Human ANG1 (Angiopoietin 1) ELISA Kit

            Human ANG1 (Angiopoietin 1) ELISA Kit

            Human ANG1 (Angiopoietin 1) ELISA Kit
            Human ANG1 (Angiopoietin 1) ELISA Kit
            Product Code : E-EL-H0007
            Brand Name : ELABSCIENCE BIOTECHN
            Product Description
            Human ANG1 (Angiopoietin 1) ELISA Kit   , PRICE@ 465US$
            Synonyms:ANG-1; ANGPT1; AGP1; AGPT

            Principle

            This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and then incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANG1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ANG1 . You can calculate the concentration of Human ANG1 in the samples by comparing the OD of the samples to the standard curve.

            Product ID E-EL-H0007
            Assay type Sandwich ELISA
            Format 96T
            Assay time 4.5h
            Reactivity Human
            Detection Method Colormetric
            Detection Range 62.500—4000.000pg/mL
            Sensitivity 37.500pg/mL
            Sample Volume Required Per Well 100μL
            Sample Type serum, plasma and other biological fluids

            Components

            Item Specifications Storage
            Micro ELISA Plate 8 wells ×12 strips 4℃/-20℃#
            Reference Standard 2 vials 4℃/-20℃#
            Reference Standard & Sample Diluent 1vial 20mL 4℃
            Concentrated Biotinylated Detection Ab 1vial 120μL 4℃/-20℃#
            Biotinylated Detection Ab Diluent 1vial 10mL 4℃
            Concentrated HRP Conjugate 1vial 120μL 4℃(shading light)
            HRP Conjugate Diluent 1vial 10mL 4℃
            Concentrated Wash Buffer (25×) 1vial 30mL 4℃
            Substrate Reagent 1vial 10mL 4℃(shading light)
             Stop Solution 1vial 10mL 4℃
            Plate Sealer 5pieces
            Manual 1 copy
            Certificate of Analysis 1 copy
            #: keep the kit at 4℃ if it’s used within 30 days, keep at -20℃ for longer storage.

            Precision

            Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Human ANG1 were tested 20 times on one plate, respectively.
            Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Human ANG1 were tested on 3 different plates, 20 replicates in each plate.


            Intra-assay Precision Inter-assay Precision
            Sample 1 2 3 1 2 3
            n 20 20 20 20 20 20
            Mean
            (pg/mL)
            216.000 498.900 1629.900 226.500 533.800 1641.800
            Standard deviation 14.300 20.000 60.300 15.400 27.200 50.900
            C V (%) 6.600 4.000 3.700 6.800 5.100 3.100



























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