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Home » Products » Elisa Kits » Human AP17(Apelin 17) ELISA Kit

Human AP17(Apelin 17) ELISA Kit

Human AP17(Apelin 17) ELISA Kit
Human AP17(Apelin 17) ELISA Kit
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Product Code : E-EL-H0236
Brand Name : Human AP17
Product Description

Intended use

This ELISA kit can be applied to the in vitro quantitative determination of Human AP17 concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Human AP17. During the reaction, Human AP17 in the sample or standard competes with a fixed amount of Human AP17 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human AP17. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human AP17 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay type Competitive
Format 96T
Assay time 2.5h
Reactivity Human
Detection Method Colormetric
Detection Range 0.16—10 ng/mL
Sensitivity 0.10 ng/mL
Sample Volume 50μL
Sample Type Serum, plasma and other biological fluids

Specificity

This kit recognizes Human AP17 in samples. No significant cross-reactivity or interference was observed.

Linearity

Samples were spiked with high concentrations of Human ACTH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 99-113 84-97 93-104
Average (%) 105 91 99
1:4 Range (%) 84-95 94-108 106
Average (%) 89 100 106
1:8 Range (%) 90-101 87-100 94-107
Average (%) 95 93 101
1:16 Range (%) 87-100 90-101 97-112
Average (%) 94 96 104

Kit components & Storage

The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.

Item Specifications Storage
Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
Reference Standard 2 vials
Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 4℃(shading light)
Stop Solution 1 vial, 10 mL 4℃
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.

Other supplies required

  • Microplate reader with 450nm wavelength filter
  • High-precision transferpettor, EP tubes and disposable pipette tips
  • 37℃ Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Loading slot for Wash Buffer

 


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