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Home » Products » Elisa Kits » Human α-CTx(Alpha Crosslaps) ELISA Kit

Human α-CTx(Alpha Crosslaps) ELISA Kit

Human α-CTx(Alpha Crosslaps) ELISA Kit
Human α-CTx(Alpha Crosslaps) ELISA Kit
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Product Code : E-EL-H0154
Brand Name : Alpha Crosslaps
Product Description

Intended use

This ELISA kit can be applied to the in vitro quantitative determination of Human α-CTx concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Human α-CTx. During the reaction, Human α-CTx in the sample or standard competes with a fixed amount of Human α-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human α-CTx. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human α-CTx in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay type Competitive
Format 96T
Assay time 2.5h
Reactivity Human
Detection Method Colormetric
Detection Range 93.75—6000 pg/mL
Sensitivity 56.25 pg/mL
Sample Volume 50μL
Sample Type Serum, plasma and other biological fluids

Specificity

This kit recognizes Human α-CTx in samples. No significant cross-reactivity or interference was observed.

Specificity

This kit recognizes Human α-CTx in samples. No significant cross-reactivity or interference was observed.

Typical data

For convenience in result calculation, absorbance can be used as ordinate and standard concentrations as abscissa. The standard curve provided in the manual is only for reference, and experimenters should draw the standard curve based on data of themselves.

(pg/mL) O.D Average
6000 0.336
0.336
0.336
3000 0.398
0.452
0.425
1500 0.6
0.578
0.589
750 0.856
0.868
0.862
375 1.256
1.23
1.243
187.5 1.672
1.654
1.663
93.75 2.012
2.024
2.018
0 2.543
2.547
2.545

 


SR GROUP
X