Item

Specifications

Micro ELISA Plate(Dismountable)

8 wells X 12 strips

Reference Standard

2 vials

Concentrated Biotinylated Detection Ab (100x)

1 vial, 120 ul

Concentrated HRP Conjugate (100x)

1 vial, 120 ul

Reference Standard & Sample Diluent

1 vial, 20 mL

Biotinylated Detection Ab Diluent

1 vial, 14 mL

HRP Conjugate Diluent

1 vial, 14 mL

Concentrated Wash Buffer (25x)

1 vial, 30 mL

Substrate Reagent

1 vial, 10 mL

Stop Solution

1 vial, 10 mL

1.Add 100 L standard or sample to each well. Incubate for 90 min at 37.

2. Remove the liquid.

3.Add 100 L Biotinylated Detection Ab. Incubate for 1 hour at 37. Aspirate and wash 3 times.

4.Add 100 L HRP Conjugate. Incubate for 30 min at 37. Aspirate and wash 5 times.

5.Add 90 L of Substrate Reagent. Incubate for 15 min at 37.

6.Add 50 L Stop Solution.

           7. Read at 450 nm immediately. Calculation of results