Product Description
Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.
Physical State: Freeze-dried solid Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Preservative: 0.05% Sodium Azide
Highly Pure, Sterile Serum for Research ApplicationsNormal Sheep Serum is trusted for its exceptional purity (>99%), sterile filtration, and low-endotoxin levels. It is especially beneficial in laboratory settings where contamination risks must be minimized, such as immunoassays, cell culture, and biotechnology research. Its sealed sterile vials ensure product integrity throughout storage and usage.
Convenient Sizing and AdaptabilityTo meet diverse laboratory needs, this serum is available in standard 10 mL, 50 mL, and 100 mL vials, as well as customizable volumes. This flexibility allows researchers to source the ideal amount for their experimental protocols, reducing waste and optimizing resource allocation.
Consistent Performance and Long Shelf LifeThe serum maintains a stable pH (7.27.4) and clarity as a clear, odorless liquid, ensuring reproducible results. With an extended shelf life of up to 18 months when stored at -20C or lower, it offers lasting reliability for ongoing and future studies.
FAQs of Normal Sheep Serum:
Q: How should Normal Sheep Serum be stored for optimal stability?
A: Normal Sheep Serum must be stored at -20C or below to maintain its pH stability and extend its shelf life up to 18 months. Ensure vials remain tightly sealed and avoid repeated freezethaw cycles to preserve product quality.
Q: What are the main uses of Normal Sheep Serum in laboratory protocols?
A: Normal Sheep Serum serves as a blocking agent in immunoassays, a control serum, and as a diluent in various research applications. It is also utilized in immunology, biotechnology, and cell culture experiments due to its high purity and low endotoxin content.
Q: When should I choose sterile, low endotoxin sheep serum over other types?
A: Sterile, low endotoxin sheep serum is ideal for sensitive assays where minimizing contamination risk is crucial, such as cell culture or immunoassays. Researchers should opt for this product when high assay reliability and reproducibility are required.
Q: Where can I order Normal Sheep Serum and in which packaging formats?
A: This product is supplied by laboratory distributors, importers, suppliers, and traders across the United States. It is available in sealed sterile vials of 10 mL, 50 mL, 100 mL and can also be ordered in custom sizes according to specific project needs.
Q: What benefits does preservative-free Normal Sheep Serum provide for research?
A: Without added preservatives, Normal Sheep Serum reduces the risk of introducing interfering substances into experiments. This is particularly advantageous in immunoassays and cell culture systems where even trace additives may skew analytical outcomes.
Q: How is the sterility and purity of the serum ensured during processing?
A: The serum is subjected to sterile filtration and rigorous quality control standards to achieve sterility and a purity level above 99%. Endotoxin testing and sealed sterile vials further guarantee that the serum meets laboratory-grade specifications.