Pregnenolone ELISA Kit

This ELISA kit can be applied to the in vitro quantitative determination of  Pregnenolone concentrations in serum, plasma and other biological fluids.

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Pregnenolone. During the reaction,  Pregnenolone in the sample or standard competes with a fixed amount of Pregnenolone on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  Pregnenolone. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of  Pregnenolone in the samples is then determined by comparing the OD of the samples to the standard curve.

This kit recognizes Pregnenolone in samples. No significant cross-reactivity or interference was observed.