Rabbit Anti-Mouse IgG (H+L)

Rabbit Anti-Mouse IgG (H+L)

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Rabbit Anti-Mouse IgG (H+L)

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MOQ : 1 Bottle

Rabbit Anti-Mouse IgG (H+L) Specification

Application
Immunofluorescence, ELISA, Western Blot, Immunohistochemistry

Smell
Odorless

Molecular Formula
Not Applicable (Protein Mixture)

Physical Form
Liquid

Molecular Weight
~150 kDa (approx. for IgG)

HS Code
3822

Shelf Life
12 to 24 months

Grade
Research Grade

Poisonous
Non-toxic (in recommended laboratory use)

Density
Gram per cubic centimeter(g/cm3)

Appearance
Liquid

Storage
Store at -20°C or below, avoid repeated freeze-thaw cycles

Usage
Detection of Mouse IgG in various immunoassays

Ph Level
~7.2

Other Names
Rabbit anti-Mouse Immunoglobulin G

Classification
Immunoglobulin

Chemical Name
Rabbit Anti-Mouse IgG (H+L)

Type
Polyclonal Antibody

Purity(%)
>98% (by SDS-PAGE)

Color
Clear or Slightly Yellow Solution

Conjugation
Unconjugated (unless specified)

Target Species
Mouse

Immunogen
Pooled normal mouse IgG

Isotype
IgG (H+L)

Formulation
Sterile filtered solution

Preservative
0.1% Sodium Azide

Host Species
Rabbit

Recommended Dilution
1:1,000 - 1:20,000 for ELISA, 1:500 - 1:5,000 for WB

Endotoxin Level
<1 EU/mg

Buffer
PBS, pH 7.2, containing 0.1% sodium azide

Specificity
Reacts with whole molecule of Mouse IgG, may cross-react with other species

About Rabbit Anti-Mouse IgG (H+L)

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.

Based on immunoelectrophoresis, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Physical State: Freeze-dried solid
Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.

Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

Purity: The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: 0.05% Sodium Azide

Key Features and Benefits

This antibody offers high specificity for mouse IgG with minimal cross-reactivity. Its polyclonal nature enhances target detection across varied applications. The products sterile and low-endotoxin formulation ensures experimental integrity, while >98% purity maximizes signal quality. Long shelf life and non-toxic formulation make it ideal for consistent research use.

Versatile Applications in Research

Rabbit Anti-Mouse IgG (H+L) is essential in immunoassays such as ELISA, Western Blotting, Immunohistochemistry, and Immunofluorescence. Its broad reactivity to whole mouse IgG molecules enables accurate detection, and the unconjugated format allows for flexible secondary labeling. Optimal dilutions help tailor experiments to specific sensitivity needs.

FAQs of Rabbit Anti-Mouse IgG (H+L):

Q: How should Rabbit Anti-Mouse IgG (H+L) be stored to ensure stability?

A: To preserve stability and extend shelf life, this antibody should be stored at 20C or colder. Avoid repeated freeze-thaw cycles, as they may compromise antibody performance and integrity.

Q: What are the recommended dilution ranges for different applications?

A: For ELISA, use a dilution of 1:1,000 to 1:20,000; for Western Blot, 1:500 to 1:5,000 is advised. Adjustments may be necessary depending on experimental requirements and sensitivity needs.

Q: When is it beneficial to use an unconjugated secondary antibody in experiments?

A: Unconjugated secondary antibodies are valuable when researchers require flexibility in subsequent detection methods or wish to incorporate their choice of conjugated secondary reagents, such as HRP, AP, or fluorophores.

Q: What is the process for using this antibody in immunoassays?

A: Dilute the antibody to the recommended concentration, depending on the assay, and use it as a secondary antibody to detect mouse IgG. Follow standard immunoassay protocols for blocking, incubation, washing, and detection steps.

Q: What benefits does the high purity (>98%) provide in research applications?

A: High purity reduces background noise and unwanted reactions, resulting in cleaner results and improved signal-to-noise ratio. This enhances the reliability and reproducibility of experimental data.

Q: Where can this antibody be sourced, and what is its typical form?

A: This research-grade antibody is available from distributors, importers, suppliers, and traders in the United States. It is supplied as a clear or slightly yellow, odorless liquid in a sterile filtered formulation.

Rabbit Anti-Mouse IgG (H+L)

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