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Home » Products » Elisa Kits » Rat CDKN1A(Cyclin Dependent Kinase Inhibitor 1A) ELISA Kit

Rat CDKN1A(Cyclin Dependent Kinase Inhibitor 1A) ELISA Kit

Rat CDKN1A(Cyclin Dependent Kinase Inhibitor 1A) ELISA Kit
Rat CDKN1A(Cyclin Dependent Kinase Inhibitor 1A) ELISA Kit
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Product Code : E-EL-R0310
Brand Name : elabscience
Product Description

Rat CDKN1A(Cyclin Dependent Kinase Inhibitor 1A) ELISA Kit

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat CDKN1A concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CDKN1A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CDKN1A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CDKN1A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CDKN1A. You can calculate the concentration of Rat CDKN1A in the samples by comparing the OD of the samples to the standard curve.

Assay type

Sandwich

Format

96T

Assay time

4.5h

Reactivity

Rat

Detection Method

Colormetric

Detection Range

78.13 5000ng/mL

Sensitivity

46.88 ng/mL

Sample Volume Required Per Well

100 ¼L

Sample Type

Serum, plasma and other biological fluids

Specificity

This kit recognizes Rat CDKN1A in samples. No significant cross-reactivity or interference between Rat CDKN1A and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.


Concentration(ng/mL)

O.D

Average

Corrected

5000

2.466
2.47

2.468

2.411

2500

1.657
1.683

1.67

1.613

1250

1.057
1.021

1.039

0.982

625

0.441
0.473

0.457

0.4

312.5

0.252
0.25

0.251

0.194

156.25

0.172
0.16

0.166

0.109

78.13

0.107
0.117

0.112

0.055

5000

2.466
2.47

2.468

2.411

Kit components & Storage

An unopened kit can be stored at 4 ƒ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item

Specifications

Storage

Micro ELISA Plate(Dismountable)

8 wells 12 strips

-20„ƒ, 6 months

Reference Standard

2 vials

Concentrated Biotinylated Detection Ab (100 )

1 vial, 120 ¼L

Concentrated HRP Conjugate (100 )

1 vial, 120 ¼L

-20„ƒ(shading light), 6 months

Reference Standard & Sample Diluent

1 vial, 20 mL

4„ƒ, 6 months

Biotinylated Detection Ab Diluent

1 vial, 14 mL

HRP Conjugate Diluent

1 vial, 14 mL

Concentrated Wash Buffer (25 )

1 vial, 30 mL

Substrate Reagent

1 vial, 10 mL

4„ƒ(shading light)

Stop Solution

1 vial, 10 mL

4„ƒ

Plate Sealer

5 pieces


Product Description

1 copy

Certificate of Analysis

1 copy




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