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Home » Products » Elisa Kits » Rat CDT(Carbohydrate Deficient Transferrin) ELISA Kit

Rat CDT(Carbohydrate Deficient Transferrin) ELISA Kit

Rat CDT(Carbohydrate Deficient Transferrin) ELISA Kit
Rat CDT(Carbohydrate Deficient Transferrin) ELISA Kit
Product Code : :E-EL-R0145
Brand Name : elabscience
Product Specifications
  • Shelf Life
  • 12 Months
Product Description

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat CDT concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CDT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CDT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CDT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CDT. You can calculate the concentration of Rat CDT in the samples by comparing the OD of the samples to the standard curve.

Assay typeSandwich
Format96T
Assay time4.5h
ReactivityRat
Detection MethodColormetric
Detection Range0.63—40 nmol/mL
Sensitivity0.38 nmol/mL
Sample Volume Required Per Well100 μL
Sample TypeSerum, plasma and other biological fluids

Specificity

This kit recognizes Rat CDT in samples. No significant cross-reactivity or interference between Rat CDT and analogues was observed.

Kit components & Storage

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells ×12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL4℃, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL4℃(shading light)
Stop Solution1 vial, 10 mL4℃
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required

  • Microplate reader with 450 nm wavelength filter
  • High-precision transfer pipette, EP tubes and disposable pipette tips
  • Incubator capable of maintaining 37℃
  • Deionized or distilled water
  • Absorbent paper
  • Loading slot for Wash Buffer
  • Recovery

    The recovery of Rat CDT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

    Sample TypeRange (%)Average Recovery (%)
    Serum (n=5)93-10799
    EDTA plasma (n=5)94-108100
    Cell culture media (n=5)85-9590

 


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