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Home » Products » Elisa Kits » Rat CENPⅠ(Centromere Protein Ⅰ) ELISA Kit

Rat CENPⅠ(Centromere Protein Ⅰ) ELISA Kit

Rat CENPⅠ(Centromere Protein Ⅰ) ELISA Kit
Rat CENPⅠ(Centromere Protein Ⅰ) ELISA Kit
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Product Code : E-EL-R0180
Brand Name : elabscience
Price And Quantity
  • Minimum Order Quantity
  • 1 kit
Product Specifications
  • Shelf Life
  • 12 Months
Product Description

Rat CENPⅠ(Centromere Protein Ⅰ) ELISA Kit 

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat CENPⅠ concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CENPⅠ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CENPⅠ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CENPⅠ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CENPⅠ. You can calculate the concentration of Rat CENPⅠ in the samples by comparing the OD of the samples to the standard curve.

Assay typeSandwich
Format96T
Assay time4.5h
ReactivityRat
Detection MethodColormetric
Detection Range0.78—50 ng/mL
Sensitivity0.47 ng/mL
Sample Volume Required Per Well100 μL
Sample TypeSerum, plasma and other biological fluids

Specificity

This kit recognizes Rat CENPⅠ in samples. No significant cross-reactivity or interference between Rat CENPⅠ and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)O.DAverageCorrected
502.467
2.467
2.4672.375
251.755
1.797
1.7761.684
12.51.035
1.015
1.0250.9330000000000001
6.250.447
0.483
0.4650.373
3.130.288
0.262
0.2750.183
1.570.206
0.188
0.1970.105
0.780.136
0.154
0.1450.053
00.091
0.093
0.092--

Kit components & Storage

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells ×12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL4℃, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL4℃(shading light)
Stop Solution1 vial, 10 mL4℃
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy

 

Other supplies required

  • Microplate reader with 450 nm wavelength filter
  • High-precision transfer pipette, EP tubes and disposable pipette tips
  • Incubator capable of maintaining 37℃
  • Deionized or distilled water
  • Absorbent paper
  • Loading slot for Wash Buffer.

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