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Home » Products » Elisa Kits » Rat CLEC2C(C-Type Lectin Domain Family 2, Member C) ELISA Kit

Rat CLEC2C(C-Type Lectin Domain Family 2, Member C) ELISA Kit

Rat CLEC2C(C-Type Lectin Domain Family 2, Member C) ELISA Kit
Rat CLEC2C(C-Type Lectin Domain Family 2, Member C) ELISA Kit
Product Code : E-EL-R0283
Brand Name : elabscience
Product Description

Rat CLEC2C(C-Type Lectin Domain Family 2, Member C) ELISA Kit

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat CLEC2C concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CLEC2C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CLEC2C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CLEC2C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CLEC2C. You can calculate the concentration of Rat CLEC2C in the samples by comparing the OD of the samples to the standard curve.

Assay type

Sandwich

Format

96T

Assay time

4.5h

Reactivity

Rat

Detection Method

Colormetric

Detection Range

31.25 2000 ng/mL

Sensitivity

18.75 ng/mL

Sample Volume Required Per Well

100 ¼L

Sample Type

Serum, plasma and other biological fluids

Specificity

This kit recognizes Rat CLEC2C in samples. No significant cross-reactivity or interference between Rat CLEC2C and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.



Concentration(ng/mL)

O.D

Average

Corrected

2000

2.536
2.554

2.545

2.478

1000

1.692
1.702

1.697

1.63

500

0.985
0.961

0.973

0.906

250

0.41
0.448

0.429

0.362

125

0.241
0.239

0.24

0.173

62.5

0.181
0.157

0.169

0.102

31.25

0.111
0.127

0.119

0.052

0

0.06
0.074

0.067

--


Kit components & Storage

An unopened kit can be stored at 4 ƒ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item

Specifications

Storage

Micro ELISA Plate(Dismountable)

8 wells 12 strips

-20„ƒ, 6 months

Reference Standard

2 vials

Concentrated Biotinylated Detection Ab (100 )

1 vial, 120 ¼L

Concentrated HRP Conjugate (100 )

1 vial, 120 ¼L

-20„ƒ(shading light), 6 months

Reference Standard & Sample Diluent

1 vial, 20 mL

4„ƒ, 6 months

Biotinylated Detection Ab Diluent

1 vial, 14 mL

HRP Conjugate Diluent

1 vial, 14 mL

Concentrated Wash Buffer (25 )

1 vial, 30 mL

Substrate Reagent

1 vial, 10 mL

4„ƒ(shading light)

Stop Solution

1 vial, 10 mL

4„ƒ

Plate Sealer

5 pieces


Product Description

1 copy

Certificate of Analysis

1 copy






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