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Home » Products » Elisa Kits » Rat COL2(Collagen Type Ⅱ) ELISA Kit

Rat COL2(Collagen Type Ⅱ) ELISA Kit

Rat COL2(Collagen Type Ⅱ) ELISA Kit
Rat COL2(Collagen Type Ⅱ) ELISA Kit
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Product Code : :E-EL-R0234
Brand Name : elabscience
Product Description

Rat COL2(Collagen Type Ⅱ) ELISA Kit

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat COL2 concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat COL2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat COL2. You can calculate the concentration of Rat COL2 in the samples by comparing the OD of the samples to the standard curve.

Assay type

Sandwich

Format

96T

Assay time

4.5h

Reactivity

Rat

Detection Method

Colormetric

Detection Range

62.50 4000 pg/mL

Sensitivity

37.50pg/mL

Sample Volume Required Per Well

100 ¼L

Sample Type

Serum, plasma and other biological fluids

Specificity

This kit recognizes Rat COL2 in samples. No significant cross-reactivity or interference between Rat COL2 and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)

O.D

Average

Corrected

4000

2.306
2.348

2.327

2.266

2000

1.581
1.589

1.585

1.524

1000

0.875
0.873

0.874

0.813

500

0.459
0.495

0.477

0.416

250

0.229
0.215

0.222

0.161

125

0.159
0.153

0.156

0.095

62.50

0.104
0.116

0.11

0.049

0

0.061
0.061

0.061

--

Kit components & Storage

An unopened kit can be stored at 4 ƒ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.


Item

Specifications

Storage

Micro ELISA Plate(Dismountable)

8 wells 12 strips

-20„ƒ, 6 months

Reference Standard

2 vials

Concentrated Biotinylated Detection Ab (100 )

1 vial, 120 ¼L

Concentrated HRP Conjugate (100 )

1 vial, 120 ¼L

-20„ƒ(shading light), 6 months

Reference Standard & Sample Diluent

1 vial, 20 mL

4„ƒ, 6 months

Biotinylated Detection Ab Diluent

1 vial, 14 mL

HRP Conjugate Diluent

1 vial, 14 mL

Concentrated Wash Buffer (25 )

1 vial, 30 mL

Substrate Reagent

1 vial, 10 mL

4„ƒ(shading light)

Stop Solution

1 vial, 10 mL

4„ƒ

Plate Sealer

5 pieces


Product Description

1 copy

Certificate of Analysis

1 copy



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