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Home » Products » Elisa Kits » Rat CRH(Corticotropin Releasing Hormone) ELISA Kit

Rat CRH(Corticotropin Releasing Hormone) ELISA Kit

Rat CRH(Corticotropin Releasing Hormone) ELISA Kit
Rat CRH(Corticotropin Releasing Hormone) ELISA Kit
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Product Code : :E-EL-R0270
Brand Name : elabscience
Product Description

Rat CRH(Corticotropin Releasing Hormone) ELISA Kit

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat CRH concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat CRH. During the reaction, Rat CRH in the sample or standard competes with a fixed amount of Rat CRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat CRH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat CRH in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay type

Sandwich

Format

96T

Assay time

2.5h

Reactivity

Rat

Detection Method

Colormetric

Detection Range

0.31 20 ng/mL

Sensitivity

0.19 ng/mL

Sample Volume Required Per Well

50 ¼L

Sample Type

Serum, plasma and other biological fluids

Specificity

This kit recognizes Rat CRH in samples. No significant cross-reactivity or interference between Rat CRH and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)

O.D

Average

20

0.351
0.359

0.355

10

0.455
0.497

0.476

5

0.704
0.668

0.686

2.5

0.996
1.012

1.004

1.25

1.392
1.388

1.39

0.63

1.752
1.75

1.751

0.31

2.019
2.037

2.028

0

2.375
2.377

2.376

Kit components & Storage

An unopened kit can be stored at 4 ƒ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item

Specifications

Storage

Micro ELISA Plate(Dismountable)

8 wells 12 strips

-20„ƒ, 6 months

Reference Standard

2 vials

Concentrated Biotinylated Detection Ab (100 )

1 vial, 120 ¼L

Concentrated HRP Conjugate (100 )

1 vial, 120 ¼L

-20„ƒ(shading light), 6 months

Reference Standard & Sample Diluent

1 vial, 20 mL

4„ƒ, 6 months

Biotinylated Detection Ab Diluent

1 vial, 14 mL

HRP Conjugate Diluent

1 vial, 14 mL

Concentrated Wash Buffer (25 )

1 vial, 30 mL

Substrate Reagent

1 vial, 10 mL

4„ƒ(shading light)

Stop Solution

1 vial, 10 mL

4„ƒ

Plate Sealer

5 pieces


Product Description

1 copy

Certificate of Analysis

1 copy




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