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Rat β-EPR(Beta-Endorphin Receptor) ELISA Kit

Rat β-EPR(Beta-Endorphin Receptor) ELISA Kit
Rat β-EPR(Beta-Endorphin Receptor) ELISA Kit
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Product Code : E-EL-R0104
Brand Name : elabscience
Product Description

Rat β-EPR(Beta-Endorphin Receptor) ELISA Kit Synonyms:b-EPR

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat β-EPR concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat β-EPR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat β-EPR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat β-EPR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat β-EPR. You can calculate the concentration of Rat β-EPR in the samples by comparing the OD of the samples to the standard curve.

Assay typeSandwich
Format96T
Assay time4.5h
ReactivityRat
Detection MethodColormetric
Detection Range31.25—2000 pg/mL
Sensitivity18.75 pg/mL
Sample Volume Required Per Well100 μL
Sample TypeSerum, plasma and other biological fluids

Specificity

This kit recognizes Rat β-EPR in samples. No significant cross-reactivity or interference between Rat β-EPR and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)O.DAverageCorrected
20002.496
2.526
2.5112.448
10001.583
1.599
1.5911.528
5000.909
0.897
0.9030.84
2500.444
0.458
0.4510.388
1250.259
0.253
0.2560.193
62.50.172
0.158
0.1650.102
31.250.111
0.119
0.1150.052
00.055
0.07099999999999999
0.063--

 

Kit components & Storage

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells ×12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL4℃, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL4℃(shading light)
Stop Solution1 vial, 10 mL4℃
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy

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