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Home » Products » Elisa Kits » Rat IL-2(Interleukin 2) ELISA Kit

Rat IL-2(Interleukin 2) ELISA Kit

Rat IL-2(Interleukin 2) ELISA Kit
Rat IL-2(Interleukin 2) ELISA Kit
Product Code : :E-EL-R0013
Brand Name : elabscience
Price And Quantity
  • Minimum Order Quantity
  • 1 kit
  • Price Range
  • 20000 - 25000
Product Specifications
  • Shelf Life
  • 12 Months
Product Description

Rat IL-2(Interleukin 2) ELISA Kit Synonyms:IL2, TCGF, Lymphokine

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat IL-2 concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-2. You can calculate the concentration of Rat IL-2 in the samples by comparing the OD of the samples to the standard curve.

Assay type Sandwich
Format 96T
Assay time 4.5h
Reactivity Rat
Detection Method Colormetric
Detection Range 31.25—2000 pg/mL
Sensitivity 18.75 pg/mL
Sample Volume Required Per Well 100 μL
Sample Type Serum, plasma and other biological fluids

Specificity

This kit recognizes Rat IL-2 in samples. No significant cross-reactivity or interference between Rat IL-2 and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL) O.D Average Corrected
2000 2.496
2.516
2.506 2.439
1000 1.66
1.666
1.663 1.596
500 0.961
0.945
0.9530000000000001 0.886
250 0.454
0.484
0.469 0.402
125 0.275
0.255
0.265 0.198
62.5 0.176
0.16
0.168 0.101
31.25 0.111
0.127
0.119 0.052
0 0.062
0.07199999999999999
0.067 --

 

Kit components & Storage

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item Specifications Storage
Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
Reference Standard 2 vials
Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 4℃(shading light)
Stop Solution 1 vial, 10 mL 4℃
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

SR GROUP

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