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Rat MMP-13(Matrix Metalloproteinase 13) ELISA Kit

Rat MMP-13(Matrix Metalloproteinase 13) ELISA Kit

18500 INR/Kit

Product Details:

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Rat MMP-13(Matrix Metalloproteinase 13) ELISA Kit Price And Quantity

  • 20000.00 - 25000.00 INR/Kit
  • 18500 INR/Kit
  • 1 Kit

Rat MMP-13(Matrix Metalloproteinase 13) ELISA Kit Product Specifications

  • 12 Months
  • ELISA Kit

Rat MMP-13(Matrix Metalloproteinase 13) ELISA Kit Trade Information

  • 500 Kit Per Day
  • 1-7 Days

Product Description

Rat MMP-13(Matrix Metalloproteinase 13) ELISA Kit Synonyms:MMP13, CLG3, MANDP1, Collagenase 3

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat MMP-13 concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Rat MMP-13. You can calculate the concentration of Rat MMP-13 in the samples by comparing the OD of the samples to the standard curve.

Assay typeSandwich
Format96T
Assay time4.5h
ReactivityRat
Detection MethodColormetric
Detection Range0.3120 ng/mL
Sensitivity0.19 ng/mL
Sample Volume Required Per Well100 L
Sample TypeSerum, plasma and other biological fluids

Specificity

This kit recognizes Rat MMP-13 in samples. No significant cross-reactivity or interference between Rat MMP-13 and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)O.DAverageCorrected
202.384
2.388
2.3862.296
101.572
1.616
1.5941.504
50.912
0.888
0.90.8100000000000001
2.50.466
0.488
0.4770.387
1.250.276
0.252
0.2640.174
0.630.196
0.182
0.1890.099
0.310.132
0.148
0.140.05
00.08699999999999999
0.093
0.09--

 

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