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Home » Products » Elisa Kits » Rat MT(Melatonin) ELISA Kit

Rat MT(Melatonin) ELISA Kit

Rat MT(Melatonin) ELISA Kit
Rat MT(Melatonin) ELISA Kit
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Product Code : E-EL-R0031
Brand Name : elabscience
Price And Quantity
  • Minimum Order Quantity
  • 1 kit
  • Price Range
  • 20000 - 25000
Product Specifications
  • Shelf Life
  • 12 Months
Product Description

Rat MT(Melatonin) ELISA Kit Synonyms:N-Acetyl-5-Methoxytryptamine

 

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat MT concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat MT. During the reaction, Rat MT in the sample or standard competes with a fixed amount of Rat MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat MT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat MT in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay typeCompetitive
Format96T
Assay time2.5h
ReactivityRat
Detection MethodColormetric
Detection Range15.63—1000 pg/mL
Sensitivity9.38 pg/mL
Sample Volume50μL
Sample TypeSerum, plasma and other biological fluids

Specificity

This kit recognizes Rat MT in samples. No significant cross-reactivity or interference between Rat MT and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)O.DAverage
10000.394
0.408
0.401
5000.483
0.527
0.505
2500.71
0.676
0.6930000000000001
1250.98
1.01
0.9949999999999999
62.51.404
1.39
1.397
31.251.826
1.812
1.819
15.632.158
2.168
2.163
02.655
2.661
2.658


Kit components & Storage

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells ×12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL4℃, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL4℃(shading light)
Stop Solution1 vial, 10 mL4℃
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy

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