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                          Home » Products » Rat VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit » Rat VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit

                          Rat VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit

                          Rat VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit
                          Rat VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit
                          Product Code : E-EL-R0130
                          Brand Name : elabscience
                          Price And Quantity
                          • Minimum Order Quantity
                          • 1 kit
                          • Price Range
                          • 20000 - 25000
                          Product Specifications
                          • Shelf Life
                          • 12 Months
                          Product Description

                          Rat VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit Synonyms:CDH5, 7B4, CD144, Cadherin 5 Type 2

                          Intended use

                          This ELISA kit applies to the in vitro quantitative determination of Rat VE-Cadherin concentrations in serum, plasma and other biological fluids.

                          Test principle

                          This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VE-Cadherin. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VE-Cadherin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VE-Cadherin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VE-Cadherin. You can calculate the concentration of Rat VE-Cadherin in the samples by comparing the OD of the samples to the standard curve.

                          Assay typeSandwich
                          Format96T
                          Assay time4.5h
                          ReactivityRat
                          Detection MethodColormetric
                          Detection Range0.16—10 ng/mL
                          Sensitivity0.10 ng/mL
                          Sample Volume Required Per Well100 μL
                          Sample TypeSerum, plasma and other biological fluids

                          Specificity

                          This kit recognizes Rat VE-Cadherin in samples. No significant cross-reactivity or interference between Rat VE-Cadherin and analogues was observed.

                          Typical data

                          As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

                          Concentration(ng/mL)O.DAverageCorrected
                          102.447
                          2.465
                          2.4562.396
                          51.625
                          1.637
                          1.6311.571
                          2.50.928
                          0.904
                          0.91599999999999990.856
                          1.250.459
                          0.461
                          0.460.4
                          0.630.26
                          0.236
                          0.2480.188
                          0.310.168
                          0.156
                          0.1620.102
                          0.160.108
                          0.12
                          0.1140.054
                          00.057
                          0.063
                          0.06--

                           

                          Kit components & Storage

                          An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

                          ItemSpecificationsStorage
                          Micro ELISA Plate(Dismountable)8 wells ×12 strips-20℃, 6 months
                          Reference Standard2 vials
                          Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
                          Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(shading light), 6 months
                          Reference Standard & Sample Diluent1 vial, 20 mL4℃, 6 months
                          Biotinylated Detection Ab Diluent1 vial, 14 mL
                          HRP Conjugate Diluent1 vial, 14 mL
                          Concentrated Wash Buffer (25×)1 vial, 30 mL
                          Substrate Reagent1 vial, 10 mL4℃(shading light)
                          Stop Solution1 vial, 10 mL4℃
                          Plate Sealer5 pieces
                          Product Description1 copy
                          Certificate of Analysis1 copy

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