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Home » Products » Elisa Kits » SEM(Nitrofurazone Metabolite) ELISA Kit

SEM(Nitrofurazone Metabolite) ELISA Kit

SEM(Nitrofurazone Metabolite) ELISA Kit
SEM(Nitrofurazone Metabolite) ELISA Kit
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Product Code : E-FS-E005
Brand Name : SEM(Nitrofurazone Me
Product Description

Test principle

This kit uses Indirect-Competitive-ELISA as the method. It can detect SEM in samples, such as honey, tissue, milk, fodder, etc. This kit is composed of MicroELISA Plate, HRP conjugate, antibody, standard and other supplementary reagents. The micro-plate provided in this kit has been pre-coated with SEM. During the reaction, SEM in the samples or standard competes with SEM on the solid phase supporter for sites of SEM antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of SEM. The concentration of SEM in the samples can be calculated by comparing the OD of the samples to the standard curve.

Technical indicator

Sensitivity: 0.05 ppb (ng/mL)

Reaction mode: 25℃, 45 min~ 15 min

Detection limit: Tissue, liver---0.1 ppb, Honey/milk/casing---0.1 ppb,

                             Milk powder/egg powder/fodder---0.1 ppb, Fish and shrimp---0.15 ppb

Cross-reactivity: Nitrofurazone metabolite---100%, Furazolidone metabolite---0.1%,

                                Nitrofurantoin metabolite---0.1%, Furaltadone metabolite---0.1%

Sample recovery rate: Tissue/liver---90%±15%,Honey/milk/casing---80%±15%; 

                                          Milk powder/egg powder/fodder--85%±25%

Kits components

Item

Specifications

Micro ELISA Plate

96 wells

Standard Liquid

1 mL each

(0ppb,0.05ppb,0.15ppb,0.45ppb,1.35ppb,4.05ppb)

High Concentration Standard (100ppb)

1 mL

Derivatization Reagent(black cap)

10 mL

HRP Conjugate (red cap)

5.5 mL

Antibody Working Solution(blue cap)

5.5 mL

Substrate Reagent A(white cap)

6 mL

Substrate Reagent B(black cap)

6 mL

Stop Solution(yellow cap )

6 mL

20×Concentrated Wash Buffer(white cap)

40 mL

2×Reconstitution Buffer (yellow cap )

50 mL

Plate Sealer

3 pieces

Sealed Bag

1 piece

Manual

1 copy

Other supplies required

Instruments: Micro-plate reader, Printer, Homogenizer, Nitrogen Evaporators, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

High-precision transferpettor: Single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).

Reagents: Ethyl acetate, N-hexane, Sodium hydroxide, Concentrated HCl, K2HPO4•3H2O, K2Fe (CN) 5(NO) ▪2H2O, ZnSO4•7H2O.


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