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Home » Products » Elisa Kits » ST/5-HT(Serotonin/5-Hydroxytryptamine) ELISA Kit

ST/5-HT(Serotonin/5-Hydroxytryptamine) ELISA Kit

ST/5-HT(Serotonin/5-Hydroxytryptamine) ELISA Kit
ST/5-HT(Serotonin/5-Hydroxytryptamine) ELISA Kit
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Product Code : E-EL-0033
Brand Name : ST/5-HT(Serotonin/5-
Product Description

Intended use

This ELISA kit can be applied to the in vitro quantitative determination of  ST/5-HT concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with ST/5-HT. During the reaction,  ST/5-HT in the sample or standard competes with a fixed amount of ST/5-HT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  ST/5-HT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  ST/5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay type Competitive
Format 96T
Assay time 2.5h
Reactivity Universal
Detection Method Colormetric
Detection Range 15.63—1000 ng/mL
Sensitivity 9.38 ng/mL
Sample Volume 50μL
Sample Type Serum, plasma and other biological fluids

 

Specificity

This kit recognizes ST/5-HT in samples. No significant cross-reactivity or interference was observed.

Typical data

For convenience in result calculation, absorbance can be used as ordinate and standard concentrations as abscissa. The standard curve provided in the manual is only for reference, and experimenters should draw the standard curve based on data of themselves.

(ng/mL) O.D Average
1000 0.402
0.426
0.414
500 0.528
0.532
0.53
250 0.73
0.722
0.726
125 1.004
1.018
1.011
62.5 1.355
1.343
1.349
31.25 1.674
1.654
1.664
15.63 1.899
1.901
1.9
0 2.217
2.221
2.219

 

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ST/5-HT were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ST/5-HT were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean
(ng/mL)
51.20 141.30 362.70 54.70 132.00 351.00
Standard
deviation
3.50 5.90 15.60 3.10 6.50 16.10
C V (%) 6.84 4.18 4.30 5.67 4.92 4.59

SR GROUP
X