This ELISA kit can be applied to the in vitro quantitative determination of Human AAA concentrations in serum, plasma and other biological fluids.
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AAA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AAA and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AAA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm Â± 2 nm. The OD value is proportional to the concentration of Human AAA. The concentration of Human AAA in samples can be calculated by comparing the OD of the samples to the standard curve.
|Detection Range||1.56â100 ng/mL|
|Sample Volume Required Per Well||100Î¼L|
|Sample Type||Serum, plasma and other biological fluids|
This kit recognizes Human AAA in samples. No significant cross-reactivity or interference was observed.
The unopened kit can be stored at 4â for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.
|Micro ELISA Plate(Dismountable)||8 wells Ã12 strips||-20â, 6 months|
|Reference Standard||2 vials|
|Concentrated Biotinylated Detection Ab (100Ã)||1 vial, 120 Î¼L|
|Concentrated HRP Conjugate (100Ã)||1 vial, 120 Î¼L||-20â(shading light), 6 months|
|Reference Standard & Sample Diluent||1 vial, 20 mL||4â, 6 months|
|Biotinylated Detection Ab Diluent||1 vial, 14 mL|
|HRP Conjugate Diluent||1 vial, 14 mL|
|Concentrated Wash Buffer (25Ã)||1 vial, 30 mL|
|Substrate Reagent||1 vial, 10 mL||4â(shading light)|
|Stop Solution||1 vial, 10 mL||4â|
|Plate Sealer||5 pieces|
|Product Description||1 copy|
|Certificate of Analysis||1 copy|
Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.
The recovery of Human AAA spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
|Sample Type||Range (%)||Average Recovery (%)|
|EDTA plasma (n=5)||87-96||93|
|Cell culture media (n=5)||0|