OQX (Olaquindox) ELI

Test principle and application

This kit uses Indirect-Competitive-ELISA as the method. It can detect OQX in samples, such as tissue, fodder, etc. This kit is composed of Micro ELISA Plate, HRP conjugate, antibody, standard and other supplementary reagents. The micro-plate provided in this kit has been pre-coated with OQX. During the reaction, OQX in the samples or standard competes with OQX on the solid phase supporter for sites of OQX antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of OQX. The concentration of OQX in the samples can be calculated by comparing the OD of the samples to the standard curve.

Technical indicator

Sensitivity: 0.5 ppb (ng/mL)

Reaction mode: 37℃, 30 min～30 min～15 min

Detection limit: Tissue---1.5 ppb    Fodder---150 ppb

Cross-reactivity: Olaquindox---100%,   Carbadox---＜0.1%

Sample recovery rate: Tissue ---80%±15%    Fodder---85%±15%

Other supplies required

Instruments: Micro-plate reader, Printer, Homogenizer, Nitrogen Evaporators, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

High-precision transferpettor: single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).

Reagents: Anhydrous acetonitrile, methyl alcohol.

Storage and valid period

Storage: Store at 2-8℃. Avoid freeze / thaw cycles.

Valid Period: 1 year, production date is on the packing box.