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Test principle and application
This kit uses Indirect-Competitive-ELISA as the method. It can detect OQX in samples, such as tissue, fodder, etc. This kit is composed of Micro ELISA Plate, HRP conjugate, antibody, standard and other supplementary reagents. The micro-plate provided in this kit has been pre-coated with OQX. During the reaction, OQX in the samples or standard competes with OQX on the solid phase supporter for sites of OQX antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of OQX. The concentration of OQX in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity: 0.5 ppb (ng/mL)
Reaction mode: 37℃, 30 min～30 min～15 min
Detection limit: Tissue---1.5 ppb Fodder---150 ppb
Cross-reactivity: Olaquindox---100%, Carbadox---＜0.1%
Sample recovery rate: Tissue ---80%±15% Fodder---85%±15%
Other supplies required
Instruments: Micro-plate reader, Printer, Homogenizer, Nitrogen Evaporators, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).
High-precision transferpettor: single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).
Reagents: Anhydrous acetonitrile, methyl alcohol.
Storage and valid period
Storage: Store at 2-8℃. Avoid freeze / thaw cycles.
Valid Period: 1 year, production date is on the packing box.