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Home » Products » Elisa Kits » AOZ (Furazolidone Metabolite) ELISA Kit

AOZ (Furazolidone Metabolite) ELISA Kit

AOZ (Furazolidone Metabolite) ELISA Kit
AOZ (Furazolidone Metabolite) ELISA Kit
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Product Code : E-FS-E003
Brand Name : AOZ (Furazolidone Me
Product Description

Test principle

This kit uses Indirect-Competitive-ELISA as the method. It can detect AOZ in samples, such as honey, fish, shrimp, birds, live, etc. This kit is composed of MicroELISA Plate, HRP conjugation, antibody, standard and other supplementary reagents. The micro-plate provided in this kit has been pre-coated with AOZ. During the reaction, AOZ in the samples or standard competes with AOZ on the solid phase supporter for sites of AOZ antibody. Then Horseradish Peroxidase (HRP) conjugation is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of AOZ. The concentration of AOZ in the samples can be calculated by comparing the OD of the samples to the standard curve.

Technical indicator

Sensitivity: 0.05 ppb (ng/mL)

Reaction mode: 25℃, 45 min~15 min

Detection limit: Tissue/liver---0.1 ppb, Honey/milk/casin---0.1 ppb,

                             Milk powder/egg powder/fodder---0.1 ppb

Cross-reactivity: Furazolidone metabolite---100%, Furaltadone metabolite---0.1%,

                                Nitrofurantoin metabolite---0.1%, Nitrofurazone metabolite---0.1%,

Sample recovery rate: Tissue/liver---80%±25%, Honey/milk/casing---75%±15%,

                                          Milk powder/egg powder/fodder---85%±25%

Kits components

Item

Specifications

Micro ELISA Plate

96 wells

Standard Liquid

1 mL each

(0 ppb,0.05 ppb,0.15 ppb,0.45 ppb,1.35 ppb,4.05 ppb)

High Concentrated Standard (100 ppb)

1 mL

Derivatization Reagent(black cap)

10 mL

HRP Conjugate (red cap)

5.5 mL

Antibody Working Solution (blue cap)

5.5 mL

Substrate Reagent A (white cap)

6 mL

Substrate Reagent B (black cap)

6 mL

Stop Solution (yellow cap)

6 mL

20×Concentrated Wash Buffer (white cap)

40 mL

2×Reconstitution Buffer (yellow cap)

50 mL

Plate Sealer

3 pieces

Sealed Bag

1 piece

Manual

1 copy

Other supplies required

Instruments: Micro-plate reader, Printer, Homogenizer, Nitrogen Evaporators, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

High-precision transferpettor: single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).

Reagents: Ethyl acetate, n-hexane, sodium hydroxide, concentrated HCl, K2HPO4•3H2O,

                    K2Fe (CN) 5(NO) •2H2O, ZnSO4•7H2O.

Storage and valid period:

Storage: Store at 2-8℃. Avoid freeze / thaw cycles.

Valid Period: 1 year, production date is on the packing box


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