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Home » Products » Elisa Kits » Human AAA(Anti-Actin Antibody) ELISA Kit

Human AAA(Anti-Actin Antibody) ELISA Kit

Human AAA(Anti-Actin Antibody) ELISA Kit
Human AAA(Anti-Actin Antibody) ELISA Kit
Product Code : E-EL-H0231
Brand Name : Anti-Actin Antibody
Product Description

Intended use

This ELISA kit can be applied to the in vitro quantitative determination of Human AAA concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AAA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AAA and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AAA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AAA. The concentration of Human AAA in samples can be calculated by comparing the OD of the samples to the standard curve.

Assay type Sandwich
Format 96T
Assay time 4.5h
Reactivity Human
Detection Method Colormetric
Detection Range 1.56—100 ng/mL
Sensitivity 0.94 ng/mL
Sample Volume Required Per Well 100μL
Sample Type Serum, plasma and other biological fluids

Specificity

This kit recognizes Human AAA in samples. No significant cross-reactivity or interference was observed.

Kit components & Storage

The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.

Item Specifications Storage
Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
Reference Standard 2 vials
Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 4℃(shading light)
Stop Solution 1 vial, 10 mL 4℃
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.

Other supplies required

  • Microplate reader with 450nm wavelength filter
  • High-precision transferpettor, EP tubes and disposable pipette tips
  • 37℃ Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Loading slot for Wash Buffer

 

Recovery

The recovery of Human AAA spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Range (%) Average Recovery (%)
Serum (n=5) 96-110 104
EDTA plasma (n=5) 87-96 93
Cell culture media (n=5)
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SR GROUP

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