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ATP Colorimetric/Fluorometric Assay Kit

ATP Colorimetric/Fluorometric Assay Kit
ATP Colorimetric/Fluorometric Assay Kit
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Product Code : SKU: K354
Brand Name : BIOVISION
Price And Quantity
  • Minimum Order Quantity
  • 1 kit
  • Unit of Measure
  • Pack/Packs
  • Price
  • 20000 INR
Product Specifications
  • Physical Form
  • Liquid
  • Taste
  • Odorless
  • Shape
  • Crystal
  • Shelf Life
  • 12 Days
  • Smell
  • Sharp
  • Usage
  • Features & Benefits • Simple procedure; takes less than 1 hour • Fast and convenient • Kit is designed to be a robust method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified.
  • Classification
  • High Purity
  • Purity(%)
  • 98
  • Color
  • BLUE BOX
Product Description

ATP Colorimetric/Fluorometric Assay Kit-100 ASSAY PRICE@ 39,000/-


Size100 assays


Detection MethodAbsorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species ReactivityAll
ApplicationsThe assay can detect as low as 50 picomol (1 µM) of ATP in various samples.
Features & Benefits• Simple procedure; takes less than 1 hour
• Fast and convenient
• Kit is designed to be a robust method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified.


Storage Conditions-20°C
Shipping ConditionsGel Pack
USAGEFor Research Use Only! Not For Use in Humans.
 Components  K354-100

ATP Assay Buffer

25 ml 
 ATP Probe (in DMSO)  0.2 ml
ATP Converter 1 vial 
Developer Mix (lyophilized) 1 vial 
ATP Standard (1 µmol; lyophilized)1 vial 

 ATP Assay Protocol:

1. Sample Preparation: Lyse 1 x 106 cells or homogenize tissues (10 mg) in 100 µl ATP Assay Buffer. Deproteinize cell lysate or tissue homogenate using Deproteinization Sample Preparation Kit  or 10 kDa Spin Column. Add 2-50 µl of sample to a 96-well plate. Adjust the volume to 50 µl/well with ATP Assay Buffer. Notes: a. As ATP is labile, for more accurate assays, we recommend using fresh samples. For samples to be assayed at later date, snap freeze samples using liquid N2 or dry ice. b. Tissues samples may contain enzymes that consume ATP rapidly. We suggest quick processing of your samples and deproteinization using Deproteinization Sample Preparation Kit (Cat. # K808). c. For unknown samples, we suggest performing a pilot experiment & testing different sample dilutions to ensure the readings are within the Standard Curve range. d. For samples having background, prepare parallel well(s) containing same amount of sample as in the test well. e. Endogenous compounds may interfere with the reaction. To ensure accurate determination of ATP in the test samples, we recommend spiking samples with a known amount of Standard (300 pmol).

2. Standard Curve Preparation: For the colorimetric assay, dilute 10 µl of the ATP Standard with 90 µl of dH2O to generate 1 mM ATP standard, mix well. Add 0, 2, 4, 6, 8, 10 µl into a series of wells and adjust volume to 50 µl/well with ATP Assay Buffer to generate 0, 2, 4, 6, 8, 10 nmol/well of ATP Standard. For the fluorometric assay, further dilute the ATP Standard to 0.01- 0.1 mM with the dH2O (Detection sensitivity is 10-100 fold higher with the fluorometric than with the colorimetric assay). Follow the procedure as for the colorimetric assay.  

3. Reaction Mix:Mix enough reagent for the number of samples and standards to be performed: For each well, prepare a total 50 µl Reaction Mix 

  Colorimetric Assay  Fluorometric Assay
 ATP Assay Buffer 44 µl  45.8 µl
ATP Probe2 µl0.2 µl* 
ATP Converter**2 µl 2 µl 
Developer2 µl 2 µl  


 

 


 

 

 

Trade Information
  • FOB Port
  • delhi
  • Payment Terms
  • Cheque
  • Supply Ability
  • All over India Per Week
  • Delivery Time
  • 10 Days
  • Sample Available
  • Yes
  • Sample Policy
  • Within a certain price range free samples are available
  • Main Domestic Market
  • All India

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