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ATP Colorimetric/Fluorometric Assay Kit

ATP Colorimetric/Fluorometric Assay Kit

20000 INR

Product Details:

  • Shape Crystal
  • Taste Odorless
  • Physical Form Liquid
  • Usage Features & Benefits Simple procedure; takes less than 1 hour Fast and convenient Kit is designed to be a robust method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified.
  • Smell Sharp
  • Shelf Life 12 Days
  • Classification High Purity
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ATP Colorimetric/Fluorometric Assay Kit Price And Quantity

  • 1 kit Pack
  • 20000 INR

ATP Colorimetric/Fluorometric Assay Kit Product Specifications

  • High Purity
  • Sharp
  • 12 Days
  • Liquid
  • Odorless
  • Features & Benefits Simple procedure; takes less than 1 hour Fast and convenient Kit is designed to be a robust method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified.
  • 98
  • Crystal
  • BLUE BOX

ATP Colorimetric/Fluorometric Assay Kit Trade Information

  • delhi
  • Cheque
  • All over India Per Week
  • 10 Days
  • Yes
  • Within a certain price range free samples are available
  • All India

Product Description

ATP Colorimetric/Fluorometric Assay Kit-100 ASSAY PRICE@ 39,000/-


Size100 assays


Detection MethodAbsorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species ReactivityAll
ApplicationsThe assay can detect as low as 50 picomol (1 M) of ATP in various samples.
Features & Benefits Simple procedure; takes less than 1 hour
Fast and convenient
Kit is designed to be a robust method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified.


Storage Conditions-20C
Shipping ConditionsGel Pack
USAGEFor Research Use Only! Not For Use in Humans.
 Components  K354-100

ATP Assay Buffer

25 ml 
 ATP Probe (in DMSO)  0.2 ml
ATP Converter 1 vial 
Developer Mix (lyophilized) 1 vial 
ATP Standard (1 mol; lyophilized)1 vial 

 ATP Assay Protocol:

1. Sample Preparation: Lyse 1 x 106 cells or homogenize tissues (10 mg) in 100 l ATP Assay Buffer. Deproteinize cell lysate or tissue homogenate using Deproteinization Sample Preparation Kit  or 10 kDa Spin Column. Add 2-50 l of sample to a 96-well plate. Adjust the volume to 50 l/well with ATP Assay Buffer. Notes: a. As ATP is labile, for more accurate assays, we recommend using fresh samples. For samples to be assayed at later date, snap freeze samples using liquid N2 or dry ice. b. Tissues samples may contain enzymes that consume ATP rapidly. We suggest quick processing of your samples and deproteinization using Deproteinization Sample Preparation Kit (Cat. # K808). c. For unknown samples, we suggest performing a pilot experiment & testing different sample dilutions to ensure the readings are within the Standard Curve range. d. For samples having background, prepare parallel well(s) containing same amount of sample as in the test well. e. Endogenous compounds may interfere with the reaction. To ensure accurate determination of ATP in the test samples, we recommend spiking samples with a known amount of Standard (300 pmol).

2. Standard Curve Preparation: For the colorimetric assay, dilute 10 l of the ATP Standard with 90 l of dH2O to generate 1 mM ATP standard, mix well. Add 0, 2, 4, 6, 8, 10 l into a series of wells and adjust volume to 50 l/well with ATP Assay Buffer to generate 0, 2, 4, 6, 8, 10 nmol/well of ATP Standard. For the fluorometric assay, further dilute the ATP Standard to 0.01- 0.1 mM with the dH2O (Detection sensitivity is 10-100 fold higher with the fluorometric than with the colorimetric assay). Follow the procedure as for the colorimetric assay.  

3. Reaction Mix:Mix enough reagent for the number of samples and standards to be performed: For each well, prepare a total 50 l Reaction Mix 

  Colorimetric Assay  Fluorometric Assay
 ATP Assay Buffer 44 l  45.8 l
ATP Probe2 l0.2 l* 
ATP Converter**2 l 2 l 
Developer2 l 2 l  


 

 


 

 

 

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