Donkey Anti-Rabbit IgG (H+L)

Donkey Anti-Rabbit IgG (H+L)

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Donkey Anti-Rabbit IgG (H+L)

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MOQ : 1 Bottle

Donkey Anti-Rabbit IgG (H+L) Specification

Shelf Life
12 months

Other Names
Donkey Anti-Rabbit Immunoglobulin G (Heavy and Light chains)

Molecular Formula
Not applicable (protein)

Grade
Research Grade

Physical Form
Liquid or lyophilized

Molecular Weight
Approx. 150 kDa

Density
Gram per cubic centimeter(g/cm3)

Appearance
Liquid or lyophilized powder

Usage
For detection of rabbit IgG in various assays

Ph Level
7.2-7.4

Smell
Odorless

HS Code
3822.00

Application
Immunohistochemistry, Western blotting, ELISA

Poisonous
No

Storage
-20°C for long term; 2-8°C for short term

Classification
Antibody

Chemical Name
Donkey Anti-Rabbit IgG (H+L)

Type
Secondary Antibody

Purity(%)
>95%

Color
Colorless to pale yellow

Storage Buffer
Varies with supplier, may include BSA or azide

Buffer
PBS, pH 7.4, may contain stabilizers and preservatives

Conjugation
Available unconjugated or conjugated to enzymes (such as HRP, AP, or fluorochromes)

Reactivity
Reacts with rabbit IgG

Species Reactivity
Rabbit

Quality Control
Checked for specificity and minimal cross-reactivity

Target
Rabbit IgG heavy and light chains (H+L)

Immunogen
Purified rabbit IgG

Sterility
Filtered through 0.2 micron filter

Formulation
Lyophilized or liquid

Host Species
Donkey

Certificate of Analysis
Available upon request

Preservative
May contain sodium azide

Hazard Class
Non-hazardous

About Donkey Anti-Rabbit IgG (H+L)

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.

Based on immunoelectrophoresis, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Physical State: Freeze-dried solid
Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.

Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

Purity: The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: 0.05% Sodium Azide

Versatile Applications

This antibody is designed for use in several immunodetection assays, including Western blotting, ELISA, and immunohistochemistry. Its compatibility with various conjugates, such as HRP or AP, allows researchers to tailor their experiments to the necessary detection methods, ensuring accurate and reliable results across different platforms.

Stringent Quality Control

Each batch undergoes rigorous testing to ensure high specificity and minimal cross-reactivity. The antibody is produced in donkeys and is carefully purified to exceed 95% purity, providing confidence in its performance for sensitive applications and minimizing background noise in assay readings.

Flexible Storage and Longevity

Packaged as either a liquid or lyophilized powder, this antibody offers extended shelf lifeup to 12 months when stored properly at -20C (long-term) or 2-8C (short-term). Its stability is further enhanced with preservatives and stabilizers included in the formulation, facilitating convenient inventory management for labs.

FAQs of Donkey Anti-Rabbit IgG (H+L):

Q: How should Donkey Anti-Rabbit IgG (H+L) be stored for optimal stability?

A: For long-term storage, keep the antibody at -20C. For short-term use, storing it at 2-8C is sufficient. Always avoid frequent freeze-thaw cycles to maintain its activity and integrity.

Q: What types of experiments can utilize this antibody?

A: This secondary antibody is suitable for several immunological assays, such as ELISA, Western blotting, and immunohistochemistry, providing sensitive and specific detection of rabbit IgG in research studies.

Q: When is it best to use the conjugated versus unconjugated forms?

A: Use the conjugated form (HRP, AP, or fluorochrome) when direct detection or visualization is required, as in colorimetric or fluorescent readouts. The unconjugated form offers flexibility for custom detection systems or secondary conjugation.

Q: Where is this antibody produced, and what quality assurances are in place?

A: This antibody is generated in donkey hosts and distributed by reputed suppliers in the United States. Each batch is rigorously tested for purity, specificity, and minimal cross-reactivity, and a certificate of analysis is available upon request.

Q: What is the benefit of using Donkey Anti-Rabbit IgG (H+L) in laboratory research?

A: It ensures high specificity in detecting rabbit antibodies, minimal cross-reactivity, and broad compatibility with various assay types, increasing experimental accuracy and reproducibility.

Q: Is the product hazardous or poisonous?

A: No, the antibody is classified as non-hazardous and odorless. However, it may contain sodium azide as a preservative, so standard laboratory precautions should be taken.

Q: What form does this antibody come in, and can it be used immediately upon arrival?

A: It is supplied as either a ready-to-use liquid or a lyophilized powder, which can be reconstituted before use. Ensure you follow the suppliers instructions for preparation and storage for optimal results.

Donkey Anti-Rabbit IgG (H+L)

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