Donkey Anti-Rabbit IgG (H+L)
9350 INR
Product Details:
- Molecular Weight Approx. 150 kDa
- Grade Research Grade
- Shelf Life 12 months
- Usage For detection of rabbit IgG in various assays
- Poisonous No
- Physical Form Liquid or lyophilized
- Smell Odorless
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Donkey Anti-Rabbit IgG (H+L) Price And Quantity
- 9350 INR
- 1 Bottle
- 9350.00 - 14250.00 INR
Donkey Anti-Rabbit IgG (H+L) Product Specifications
- -20C for long term; 2-8C for short term
- Odorless
- >95%
- Liquid or lyophilized
- No
- Gram per cubic centimeter(g/cm3)
- For detection of rabbit IgG in various assays
- Research Grade
- 12 months
- Approx. 150 kDa
- Liquid or lyophilized powder
- 7.2-7.4
- Donkey Anti-Rabbit IgG (H+L)
- Not applicable (protein)
- Donkey Anti-Rabbit Immunoglobulin G (Heavy and Light chains)
- Antibody
- Secondary Antibody
- 3822.00
- Colorless to pale yellow
- Immunohistochemistry, Western blotting, ELISA
Product Description
Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.
Based on immunoelectrophoresis, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
Physical State: Freeze-dried solidStorage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Purity: The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: 0.05% Sodium Azide
Versatile Applications
This antibody is designed for use in several immunodetection assays, including Western blotting, ELISA, and immunohistochemistry. Its compatibility with various conjugates, such as HRP or AP, allows researchers to tailor their experiments to the necessary detection methods, ensuring accurate and reliable results across different platforms.
Stringent Quality Control
Each batch undergoes rigorous testing to ensure high specificity and minimal cross-reactivity. The antibody is produced in donkeys and is carefully purified to exceed 95% purity, providing confidence in its performance for sensitive applications and minimizing background noise in assay readings.
Flexible Storage and Longevity
Packaged as either a liquid or lyophilized powder, this antibody offers extended shelf lifeup to 12 months when stored properly at -20C (long-term) or 2-8C (short-term). Its stability is further enhanced with preservatives and stabilizers included in the formulation, facilitating convenient inventory management for labs.
FAQs of Donkey Anti-Rabbit IgG (H+L):
Q: How should Donkey Anti-Rabbit IgG (H+L) be stored for optimal stability?
A: For long-term storage, keep the antibody at -20C. For short-term use, storing it at 2-8C is sufficient. Always avoid frequent freeze-thaw cycles to maintain its activity and integrity.Q: What types of experiments can utilize this antibody?
A: This secondary antibody is suitable for several immunological assays, such as ELISA, Western blotting, and immunohistochemistry, providing sensitive and specific detection of rabbit IgG in research studies.Q: When is it best to use the conjugated versus unconjugated forms?
A: Use the conjugated form (HRP, AP, or fluorochrome) when direct detection or visualization is required, as in colorimetric or fluorescent readouts. The unconjugated form offers flexibility for custom detection systems or secondary conjugation.Q: Where is this antibody produced, and what quality assurances are in place?
A: This antibody is generated in donkey hosts and distributed by reputed suppliers in the United States. Each batch is rigorously tested for purity, specificity, and minimal cross-reactivity, and a certificate of analysis is available upon request.Q: What is the benefit of using Donkey Anti-Rabbit IgG (H+L) in laboratory research?
A: It ensures high specificity in detecting rabbit antibodies, minimal cross-reactivity, and broad compatibility with various assay types, increasing experimental accuracy and reproducibility.Q: Is the product hazardous or poisonous?
A: No, the antibody is classified as non-hazardous and odorless. However, it may contain sodium azide as a preservative, so standard laboratory precautions should be taken.Q: What form does this antibody come in, and can it be used immediately upon arrival?
A: It is supplied as either a ready-to-use liquid or a lyophilized powder, which can be reconstituted before use. Ensure you follow the suppliers instructions for preparation and storage for optimal results.Tell us about your requirement
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Contact Details
- Plot No- D17/103, Rohini Sector 3, Near Ram Lila Park, Delhi - 110085, India
- Phone :08045816517
- Send Inquiry
Send Inquiry- Mr Rambhajan Dwivedi (Proprietor)
- Mobile :08045816517
- Customer Care Number---011-27511805
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