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Human ADP/Acrp30 (Adiponectin) ELISA Kit

Human ADP/Acrp30 (Adiponectin) ELISA Kit
Human ADP/Acrp30 (Adiponectin) ELISA Kit
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Product Code : E-EL-H0004
Brand Name : ELABSCIENCE BIOTECHN
Product Description
Human ADP/Acrp30 (Adiponectin) ELISA Kit  PRICE @ 250 $ AND 70$ SHIPPING CHARGE EXTRA
Synonyms:GBP28; ACDC; APM1; ADPN; AdipoQ; ADIPQTL1

Principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADP/Acrp30 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and then incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADP/Acrp30 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ADP/Acrp30 . You can calculate the concentration of Human ADP/Acrp30 in the samples by comparing the OD of the samples to the standard curve.

Product ID E-EL-H0004
Assay type Sandwich ELISA
Format 96T
Assay time 4.5h
Reactivity Human
Detection Method Colormetric
Detection Range 0.780—50.000ng/mL
Sensitivity 0.470ng/mL
Sample Volume Required Per Well 100μL
Sample Type serum, plasma and other biological fluids

Components

Item Specifications Storage
Micro ELISA Plate 8 wells ×12 strips 4℃/-20℃#
Reference Standard 2 vials 4℃/-20℃#
Reference Standard & Sample Diluent 1vial 20mL 4℃
Concentrated Biotinylated Detection Ab 1vial 120μL 4℃/-20℃#
Biotinylated Detection Ab Diluent 1vial 10mL 4℃
Concentrated HRP Conjugate 1vial 120μL 4℃(shading light)
HRP Conjugate Diluent 1vial 10mL 4℃
Concentrated Wash Buffer (25×) 1vial 30mL 4℃
Substrate Reagent 1vial 10mL 4℃(shading light)
 Stop Solution 1vial 10mL 4℃
Plate Sealer 5pieces
Manual 1 copy
Certificate of Analysis 1 copy

Precision-Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Human ADP/Acrp30 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Human ADP/Acrp30 were tested on 3 different plates, 20 replicates in each plate.


Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean
(ng/mL)
2.400 5.800 24.900 2.300 6.300 25.900
Standard deviation 0.100 0.300 1.100 0.200 0.300 0.900
C V (%) 5.800 5.600 4.500 6.900 5.000 3.600

Recovery

The recovery of Human ADP/Acrp30 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Range (%) Average Recovery (%)
Serum (n=5) 89-104 95.000
EDTA plasma (n=5) 91-103 98.000
Cell culture media (n=5) 94-109 100.000



























Specificity

This kit recognizes recombinant and natural Human ADP/Acrp30 . No significant cross-reactivity or interference was observed.

Procedure

  1. Add 100μL standard or sample to each well. Incubate 90 mintues at 37℃.
  2. Add 100μL Biotinylated Detection Ab. Incubate 1 hour at 37℃.
  3. Aspirate and wash 3 times.
  4. Add 100μL HRP Conjugate. Incubate 30 minutes at 37℃.
  5. Aspirate and wash 5 times.
  6. Add 90μL Substrate Reagent. Incubate 15 minutes at 37℃.
  7. Add 50μL Stop Solution. Read it at 450nm immediately.
  8. Calculation of results.


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