Rat BK(Bradykinin) ELISA Kit

Rat BK(Bradykinin) ELISA Kit

18500 INR/Kit

Product Details:


Rat BK(Bradykinin) ELISA Kit Price And Quantity

  • 15000.00 - 25000.00 INR/Kit
  • 1 Kit
  • 18500 INR/Kit

Rat BK(Bradykinin) ELISA Kit Product Specifications

  • ELISA Kit

Rat BK(Bradykinin) ELISA Kit Trade Information

  • 500 Kit Per Day
  • 1-7 Days

Product Description

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat BK concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat BK. During the reaction, Rat BK in the sample or standard competes with a fixed amount of Rat BK on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat BK. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Rat BK in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay typeCompetitive
Assay time2.5h
Detection MethodColormetric
Detection Range1.2580 ng/mL
Sensitivity0.75 ng/mL
Sample Volume50L
Sample TypeSerum, plasma and other biological fluids


This kit recognizes Rat BK in samples. No significant cross-reactivity or interference between Rat BK and analogues was observed.

Kit components & Storage

An unopened kit can be stored at 4 for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Micro ELISA Plate(Dismountable)8 wells 12 strips-20, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100)1 vial, 120 L
Concentrated HRP Conjugate (100)1 vial, 120 L-20(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL4, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL4(shading light)
Stop Solution1 vial, 10 mL4
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required

  • Microplate reader with 450 nm wavelength filter
  • High-precision transfer pipette, EP tubes and disposable pipette tips
  • Incubator capable of maintaining 37
  • Deionized or distilled water
  • Absorbent paper
  • Loading slot for Wash Buffer


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