This ELISA kit can be applied to the in vitro quantitative determination of 15d-PGJ2 concentrations in serum, plasma and other biological fluids.
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 15d-PGJ2. During the reaction, 15d-PGJ2 in the sample or standard competes with a fixed amount of 15d-PGJ2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 15d-PGJ2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of 15d-PGJ2 in the samples is then determined by comparing the OD of the samples to the standard curve.
|Detection Range||3.13200 ng/mL|
|Sample Type||Serum, plasma and other biological fluids|
This kit recognizes 15d-PGJ2 in samples. No significant cross-reactivity or interference was observed.
The unopened kit can be stored at 4 for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.
|Micro ELISA Plate(Dismountable)||8 wells 12 strips||-20, 6 months|
|Reference Standard||2 vials|
|Concentrated Biotinylated Detection Ab (100)||1 vial, 120 L|
|Concentrated HRP Conjugate (100)||1 vial, 120 L||-20(shading light), 6 months|
|Reference Standard & Sample Diluent||1 vial, 20 mL||4, 6 months|
|Biotinylated Detection Ab Diluent||1 vial, 14 mL|
|HRP Conjugate Diluent||1 vial, 14 mL|
|Concentrated Wash Buffer (25)||1 vial, 30 mL|
|Substrate Reagent||1 vial, 10 mL||4(shading light)|
|Stop Solution||1 vial, 10 mL||4|
|Plate Sealer||5 pieces|
|Product Description||1 copy|
|Certificate of Analysis||1 copy|
Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.
1.Add 50 L of standard or sample to each well.
2.Immediately add 50 L of Biotinylated Detection Ab to each well.
3.Incubate for 45 min at 37. Aspirate and wash 3 times.
4.Add 100 L of HRP Conjugate to each well. Incubate for 30 min at 37. Aspirate and wash 5 times.
5.Add 90 L of Substrate Reagent. Incubate for 15 min at 37.
6.Add 50 L of Stop Solution.
7.Read the OD at 450 nm immediately. Calculation of the results.
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