
This ELISA kit can be applied to the in vitro quantitative determination of 6-keto-PGF1a concentrations in serum, plasma and other biological fluids.
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 6-keto-PGF1a. During the reaction, 6-keto-PGF1a in the sample or standard competes with a fixed amount of 6-keto-PGF1a on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 6-keto-PGF1a. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 6-keto-PGF1a in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay type | Competitive |
Format | 96T |
Assay time | 2.5h |
Reactivity | Universal |
Detection Method | Colormetric |
Detection Range | 15.63—1000 pg/mL |
Sensitivity | 9.38 pg/mL |
Sample Volume | 50μL |
Sample Type | Serum, plasma and other biological fluids |
This kit recognizes 6-keto-PGF1a in samples. No significant cross-reactivity or interference was observed.
The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 8 wells ×12 strips | -20℃, 6 months |
Reference Standard | 2 vials | |
Concentrated Biotinylated Detection Ab (100×) | 1 vial, 120 μL | |
Concentrated HRP Conjugate (100×) | 1 vial, 120 μL | -20℃(shading light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 4℃, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 4℃(shading light) |
Stop Solution | 1 vial, 10 mL | 4℃ |
Plate Sealer | 5 pieces | |
Product Description | 1 copy | |
Certificate of Analysis | 1 copy |
Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.
1.Add 50 μL of standard or sample to each well. | |
2.Immediately add 50 μL of Biotinylated Detection Ab to each well. | |
3.Incubate for 45 min at 37℃. Aspirate and wash 3 times. | |
4.Add 100 μL of HRP Conjugate to each well. Incubate for 30 min at 37℃. Aspirate and wash 5 times. | |
5.Add 90 μL of Substrate Reagent. Incubate for 15 min at 37℃. | |
6.Add 50 μL of Stop Solution. | |
7.Read the OD at 450 nm immediately. Calculation of the results. |
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