call-us

8-epi-PGF2(8-Epi-Prostaglandin F2 Alpha) ELISA Kit

8-epi-PGF2(8-Epi-Prostaglandin F2 Alpha) ELISA Kit
8-epi-PGF2(8-Epi-Prostaglandin F2 Alpha) ELISA Kit
Send Inquiry
Product Code : E-EL-0041
Brand Name : Epi-Prostaglandin F2
Product Description

Intended use

This ELISA kit can be applied to the in vitro quantitative determination of  8-epi-PGF2 concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 8-epi-PGF2. During the reaction,  8-epi-PGF2 in the sample or standard competes with a fixed amount of 8-epi-PGF2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  8-epi-PGF2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of  8-epi-PGF2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay type Competitive
Format 96T
Assay time 2.5h
Reactivity Universal
Detection Method Colormetric
Detection Range 15.631000 pg/mL
Sensitivity 9.38 pg/mL
Sample Volume 50L
Sample Type Serum, plasma and other biological fluids

Specificity

This kit recognizes 8-epi-PGF2 in samples. No significant cross-reactivity or interference was observed.

Kit components & Storage

The unopened kit can be stored at 4 for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.

Item Specifications Storage
Micro ELISA Plate(Dismountable) 8 wells 12 strips -20, 6 months
Reference Standard 2 vials
Concentrated Biotinylated Detection Ab (100) 1 vial, 120 L
Concentrated HRP Conjugate (100) 1 vial, 120 L -20(shading light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 4, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 4(shading light)
Stop Solution 1 vial, 10 mL 4
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.

 
















SR GROUP
X


SR GROUP All Rights Reserved.(Terms of Use)
Developed and Managed by Infocom Network Private Limited.