Am (Amantadine) ELISA Kit

Test principle

This kit uses Indirect-Competitive-ELISA as the method. It can detect tissue, such as beef, chicken, pork, etc. This kit is composed of Micro ELISA Plate, HRP conjugate, antibody, standard and other supplementary reagents. The microplate provided in this kit has been pre-coated with Am (Amantadine). During the test, Am in the samples or standard competes with Amantadine on the solid phase supporter for sites of Am antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Am. The concentration of Am in the sample.

Technical indicator

• Sensitivity: 0.5 ppb (ng/mL)
• Reaction mode: 25℃, 30 min~ 30 min~ 15 min
• Detection limit: Tissue (beef, chicken, pork)---0.5 ppb, 
• Cross-reactivity: Amantadine---100%, Amoxicillin---＜0.1%,Ceftiofur---＜0.1%,
• Sample recovery rate: Tissue---90%±20%

Kits components

Other supplies required

Instruments: Micro-plate reader, Printer, Homogenizer, Nitrogen Evaporators, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

High-precision transferpettor: single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).Reagents: Acetonitrile, Deionized water, sodium sulphate anhydrous.

Storage and valid period

Storage: Store at 2-8℃. Avoid freeze / thaw cycles.

Valid Period: 1 year, production date is on the packing box.