Am (Amantadine) ELISA Kit

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Product Code : E-FS-E006

Brand Name : Am (Amantadine) ELIS

Product Description

Test principle

This kit uses Indirect-Competitive-ELISA as the method. It can detect tissue, such as beef, chicken, pork, etc. This kit is composed of Micro ELISA Plate, HRP conjugate, antibody, standard and other supplementary reagents. The microplate provided in this kit has been pre-coated with Am (Amantadine). During the test, Am in the samples or standard competes with Amantadine on the solid phase supporter for sites of Am antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Am. The concentration of Am in the sample.

Technical indicator

Sensitivity: 0.5 ppb (ng/mL)
Reaction mode: 25℃, 30 min~ 30 min~ 15 min
Detection limit: Tissue (beef, chicken, pork)---0.5 ppb,
Cross-reactivity: Amantadine---100%, Amoxicillin---＜0.1%,Ceftiofur---＜0.1%,
Sample recovery rate: Tissue---90%±20%

Kits components

Item	Specifications
Micro ELISA Plate	96 wells
Standard Liquid	1 mL each (0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb, 40.5 ppb)
High Concentrated Standard (100 ppb)	1 mL
HRP Conjugate (red cap)	11 mL
Antibody Working Solution (blue cap)	5.5 mL
Substrate Reagent A (white cap)	6 mL
Substrate Reagent B (black cap)	6 mL
Stop Solution(yellow cap )	6 mL
20×Concentrated Wash Buffer (white cap)	40 mL
2×Reconstitution Buffer (yellow cap )	50 mL
Plate Sealer	3 pieces
Sealed Bag	1 piece
Manual	1 copy

Other supplies required

Instruments: Micro-plate reader, Printer, Homogenizer, Nitrogen Evaporators, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

High-precision transferpettor: single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).Reagents: Acetonitrile, Deionized water, sodium sulphate anhydrous.

Storage and valid period

Storage: Store at 2-8℃. Avoid freeze / thaw cycles.

Valid Period: 1 year, production date is on the packing box.