Anti-His Tag (HIS.H8) Monoclonal Antibody 100 gr

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Product Code : T001

Brand Name : EPITOPE BIOTECH

Price And Quantity

Minimum Order Quantity
100 ug Pack
Price Range
7000.00 - 20000.00 INR

Price
13000 INR

Product Specifications

Molecular Formula
not applicable
Appearance
white

Physical Form
Liquid
Usage
Detection of 6 X His Tag by Westorn Blotting EBI's Anti-His Tag (HIS.H8) mAb is one of the most popular anti-His tag Ab on the global market. We estimate that thousands of labs around the world use it. Numerous successful papers published in top scientific journals serve to showcase the high sensitivity and high affinity of this mAb

Other Names
Anti-His Tag (HIS.H8) Monoclonal Antibody
Taste
Odorless

Smell
Stimulus
Shelf Life
24 Months

Shape
lyophilized
Classification
Antibody

Chemical Name
Proteins
CAS No
T001

Type
Monoclonal antibody
Purity(%)
100% WB (with ECL): 1-2ng of His-tagged fusion proteins

Color
white

Product Description

Anti-His Tag (HIS.H8) Monoclonal Antibody

Anti-His Tag (HIS.H8) mAb is one of the most popular anti-His tag Ab on the global market. We estimate that thousands of labs around the world use it. Numerous successful papers published in top scientific journals serve to showcase the high sensitivity and high affinity of this mAb

To Order

Catalog No.

T001

E-mail

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Unit Size Pack and Price

Tel (Delhi)	011-27511805

pack size	price
50 ug Lyophilize	8,000/-
100 ug Lyophil	13,000/-
50 ug liquid	9,000/-
100 ug liquid	14000/-

Mobile (India )	9990521622

Description	Anti-His Tag (HIS.H8) Monoclonal Antibody
Clonality	HIS.H8
Host Species	Mouse
Isotype	IgG2b
Immunogen	HHHHHH (6x His) synthetic peptide conjugated to KLH.
Specificity	Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags).
Purification	Protein A affinity chromatography from mouse ascites fluid.
Applications	Dot, ELISA, IP, IS, WB (see notations).
Sensitivity	WB (with ECL): 1-2ng of His-tagged fusion proteins.
Form	Liquid: 1mg/mL in 10mM PBS, pH 7.2, 0.05% NaN₃. Lyophilized: 1mg/mL lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% NaN₃.
Working Conditions	WB (with ECL): 1:1,000-3,000 dilution (incubate for one hour at room temperature). IS: 1:500-2000 dilution. For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Storage	Liquid: Store at -20Ã‚Â°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4Ã‚Â°C for several days to weeks. Lyophilized: Store at -20Ã‚Â°C for 3 years or more. Reconstitute with distilled water to desired concentration before use. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4Ã‚Â°C for several days to weeks Product Citations- Hertz, R. et al. 2014. The Entamoeba histolytica Dnmt2 homolog (Ehmeth) confers resistance to nitrosative stress. Eukaryotic cell Mino, K. et al. 2014. Regulation of tissue factor pathway inhibitor-2 (TFPI-2) expression by lysine-specific demethylase 1 and 2 (LSD1 and LSD2). Biosci. Biotechnol. Biochem.. 78: 1010-7