AOZ (Furazolidone Metabolite) ELISA Kit

About AOZ (Furazolidone Metabolite) ELISA Kit

Test principle

This kit uses Indirect-Competitive-ELISA as the method. It can detect AOZ in samples, such as honey, fish, shrimp, birds, live, etc. This kit is composed of MicroELISA Plate, HRP conjugation, antibody, standard and other supplementary reagents. The micro-plate provided in this kit has been pre-coated with AOZ. During the reaction, AOZ in the samples or standard competes with AOZ on the solid phase supporter for sites of AOZ antibody. Then Horseradish Peroxidase (HRP) conjugation is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of AOZ. The concentration of AOZ in the samples can be calculated by comparing the OD of the samples to the standard curve.

Technical indicator

Sensitivity: 0.05 ppb (ng/mL)

Reaction mode: 25, 45 min~15 min

Detection limit: Tissue/liver---0.1 ppb, Honey/milk/casin---0.1 ppb,

                             Milk powder/egg powder/fodder---0.1 ppb

Cross-reactivity: Furazolidone metabolite---100%, Furaltadone metabolite---0.1%,

                                Nitrofurantoin metabolite---0.1%, Nitrofurazone metabolite---0.1%,

Sample recovery rate: Tissue/liver---80%25%, Honey/milk/casing---75%15%,

                                          Milk powder/egg powder/fodder---85%25%

Kits components

Other supplies required

Instruments: Micro-plate reader, Printer, Homogenizer, Nitrogen Evaporators, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

High-precision transferpettor: single channel (20-200 L, 100-1000 L), Multichannel (300 L).

Reagents: Ethyl acetate, n-hexane, sodium hydroxide, concentrated HCl, K2HPO43H2O,

                    K2Fe (CN) 5(NO) 2H2O, ZnSO47H2O.

Storage and valid period:

Storage: Store at 2-8. Avoid freeze / thaw cycles.

Valid Period: 1 year, production date is on the packing box