Caspase-3 Colorimetric Assay Kit 25 test Price @13500/- 100 test price @29,000

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Product Code : K106

Brand Name : BIOVISION USA

Price And Quantity

Minimum Order Quantity
1 kit Box
Price
13000 INR

Price Range
13000.00 - 50000.00 INR

Product Specifications

Shape
RECTANGULAR
Other Names
CASPASE 3 ASSAY COLORIMETRY

Usage
Caspases (cysteine-aspartic proteases, cysteine aspartases or cysteine-dependent aspartate-directed proteases) are a family of protease enzymes playing essential roles in programmed cell death (including apoptosis, pyroptosis and necroptosis) and inflammation.
Smell
Stimulus

Shelf Life
12 Months
Taste
Odorless

Appearance
BOX RED COLOR
Physical Form
Liquid

Classification
APOPTOSIS
Chemical Name
CASPASE 3 ASSAY

CAS No
K106
Type
ASSAY KIT CANCER RESEARCH(APOPTOSIS)

Purity(%)
100 PERCENT SENSITIVITY
EINECS No
NOT APPLICABLE

Color
RED COLOR BOX

Product Description

Caspase-3 Colorimetric Assay Kit Product code K106 price @ 13,000- 39,000/-Tax 5%

How To Order

email us - or Call us mobile/ whatsapp- +91-9990521622- 01127511805

Packing-

Cat no	size	price	status
K106-25	25 test	13000/-	In stock
K106-100	100 test	29,000/-	In stock
K106 -200	200 Test	48,000/-	10-12 Days

Kit Principal- Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.

Detection MethodAbsorbance (400 or 405 nm)Species ReactivityMammalianApplicationsDetect early/middle stages of apoptosis; differentiate apoptosis from necrosis.Features & Benefitsâ¢ Simple one-step procedure; takes 1-2 hours
â¢ Fast and convenient
â¢ Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.Kit Componentsâ¢ Cell Lysis buffer
â¢ 2X Reaction Buffer
â¢ DEVD-pNA (4 mM)
â¢ DTT (1 M)
â¢ Dilution BufferStorage Conditions-20Â°CShipping ConditionsGel PackUSAGE

For Research Use Only! Not For Use in Humans

Product Citations-

Anderson, E. J. et. al. Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol, Jan 2011; 300: H118 - H124.

Baker et al., Molecular and Cellular Mechanisms Responsible for Cellular Stress and Low-grade Inflammation Induced by a Super-low Dose of Endotoxin. J. Biol. Chem., Jun 2014; 289: 16262 - 16269.

Carley Glass et. al. MicroRNA-1 transfected embryonic stem cells enhance cardiac myocyte differentiation and inhibit apoptosis by modulating the PTEN/Akt pathway in the infarcted heart. Am J Physiol Heart Circ Physiol, Nov 2011; 301: H2038 - H2049

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