Introduction

Guanosine 3, 5-cyclic monophosphate (cGMP) is a key second messenger molecule in intracellular signal transduction. Monitoring cGMP levels is one of the most common ways to screen for agonists and antagonists of G proteincoupled receptors (GPCRs).

Elabscience cGMP ELISA Kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with goat anti-mouse IgG. During the reaction, sample or standard, cGMP-HRP Conjugate and mouse monoclonal anti-cGMP antibody (cGMP MAb) are sequentially added to each wells. cGMP in the sample or standard competes with a fixed amount of HRP-labeled cGMP for the binding sites of cGMP MAb. Free components are washed from the plate. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of cGMP in the samples is then determined by comparing the OD of the samples to the standard curve. The ELISA typically displays an IC50 (50% B/B0) of approximately 5 pmol/mL and a detection limit of approximately 0.1 pmol/mL.

Kit components