Send Email Email
Cotinine ELISA Kit
Cotinine ELISA Kit
images Send Inquiry
Request To Call Back

Cotinine ELISA Kit

Price 18500 INR/ Kit

Get a Price/Quote
MOQ : 1 Piece

Cotinine ELISA Kit Specification

  • Type
  • ELISA Kit
 

Cotinine ELISA Kit Trade Information

  • Minimum Order Quantity
  • 1 Piece
  • Supply Ability
  • 500 Pieces Per Day
  • Delivery Time
  • 1-7 Days
 

About Cotinine ELISA Kit

Intended use

This ELISA kit can be applied to the in vitro quantitative determination of  Cotinine concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Cotinine. During the reaction,  Cotinine in the sample or standard competes with a fixed amount of Cotinine on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  Cotinine. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of  Cotinine in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay type Competitive
Format 96T
Assay time 2.5h
Reactivity Universal
Detection Method Colormetric
Detection Range 0.6340 ng/mL
Sensitivity 0.38 ng/mL
Sample Volume 50L
Sample Type Serum, plasma and other biological fluids

Specificity

This kit recognizes Cotinine in samples. No significant cross-reactivity or interference was observed.

Kit components & Storage

The unopened kit can be stored at 4 for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.

Item Specifications Storage
Micro ELISA Plate(Dismountable) 8 wells 12 strips -20, 6 months
Reference Standard 2 vials
Concentrated Biotinylated Detection Ab (100) 1 vial, 120 L
Concentrated HRP Conjugate (100) 1 vial, 120 L -20(shading light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 4, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 4(shading light)
Stop Solution 1 vial, 10 mL 4
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.

Other supplies required

  • Microplate reader with 450nm wavelength filter
  • High-precision transferpettor, EP tubes and disposable pipette tips
  • 37 Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Loading slot for Wash Buffer

Assay Procedure

1.Add 50 L of standard or sample to each well.

2.Immediately add 50 L of Biotinylated Detection Ab to each well.

3.Incubate for 45 min at 37. Aspirate and wash 3 times.

4.Add 100 L of HRP Conjugate to each well. Incubate for 30 min at 37. Aspirate and wash 5 times.

5.Add 90 L of Substrate Reagent. Incubate for 15 min at 37.

6.Add 50 L of Stop Solution.

7.Read the OD at 450 nm immediately. Calculation of the results.

 

Cotinine ELISA Kit
Tell us about your requirement
product

Price:

Quantity
Select Unit

  • 50
  • 100
  • 200
  • 250
  • 500
  • 1000+
Additional detail
Mobile number

Email

More Products in Elisa Kits Category

Mouse APOB(Apolipoprotein B) ELISA Kit

Mouse APOB(Apolipoprotein B) ELISA Kit

Price 25750.0 INR / Piece

Minimum Order Quantity : 1 Piece

Type : ELISA Kit

Shelf Life : 612 Months

Ph Level : Kit buffers typically pH 7.27.4

HS Code : 3822.00

Get a Price/Quote
Mouse APOM(Apolipoprotein M) ELISA Kit

Mouse APOM(Apolipoprotein M) ELISA Kit

Price 25750.0 INR / Piece

Minimum Order Quantity : 1 Piece

Type : Biochemical Kit

Shelf Life : 612 Months

Ph Level : For best performance, follow manufacturers protocol. Usually reagents/Buffers are pH 7.27.4

HS Code : 3822

Get a Price/Quote
Pig RBP4(Retinol Binding Protein 4, Plasma) ELISA Kit

Pig RBP4(Retinol Binding Protein 4, Plasma) ELISA Kit

Price 25750.0 INR / Piece

Minimum Order Quantity : 1 Piece

Type : ELISA Kit

Shelf Life : 612 months from manufacture (refer to component labels)

Ph Level : Depends on included buffer; generally 7.27.4

HS Code : 38220090

Get a Price/Quote
Rat LH(Luteinizing Hormone) ELISA Kit

Rat LH(Luteinizing Hormone) ELISA Kit

Price 18500 INR / Kit

Minimum Order Quantity : 1 Piece

Type : ELISA Kit

Shelf Life : 12 Months

Get a Price/Quote


Contact Details

    GST : 07BXSPD1989H1ZY

  • Plot No- D17/103, Rohini Sector 3, Near Ram Lila Park, Delhi - 110085, India
  • Customer Care Number---011-27511805
  • Mr Rambhajan Dwivedi (Proprietor)
  • Mobile : 08045816517
  • Phone : 08045816517
Send Inquiry Send Inquiry
Send SMS Send SMS
  • trusted seller
  • Quick Links
    Our Products
    View All
    Back to top
    Tradeindia
    SR GROUP All Rights Reserved.(Terms of Use)
    Developed and Managed by Infocom Network Private Limited.