FAQ's of Dog PRL(Prolactin) ELISA Kit:
Q: How does the Dog PRL ELISA Kit achieve high sensitivity and precision?
A: The kit employs a sandwich ELISA assay principle, combined with a colorimetric detection method at 450 nm, ensuring highly sensitive detection (down to 0.061 ng/mL) with intra-assay CV less than 10% and inter-assay CV less than 12% for reliable results.
Q: What types of samples can be used with this ELISA kit?
A: You can use serum, plasma, and other biological fluids for analysis with this kit, making it versatile for various research and diagnostic requirements involving canine prolactin measurement.
Q: When should I order the Dog PRL ELISA Kit considering availability?
A: As these kits are supplied in limited stock, it is advisable to order promptly to ensure availability for your ongoing or upcoming research or diagnostic needs.
Q: Where should the kit be stored after receiving it?
A: Upon receipt, the Dog PRL ELISA Kit should be stored at 2-8C and must not be frozen to maintain reagent stability and effectiveness throughout its 6-12 month shelf life.
Q: What is the process for conducting the assay using this kit?
A: The process involves adding 50-100 L sample per well onto the pre-coated microplate, applying standards and reagents, incubating approximately 2.5-3.5 hours, and measuring absorbance at 450 nm. Full instructions are provided in the included manual.
Q: What are the main benefits of using this ELISA kit for research?
A: The main benefits include optimized high-sensitivity detection, quantitative analysis across a physiologically relevant range, robust performance, user-friendly protocol, and components compatible with canine biological samples.