FAQ's of E2(Estradiol) ELISA Kit:
Q: How is the E2 (Estradiol) ELISA Kit used in research laboratories?
A: The kit is applied to quantitatively measure estradiol levels in serum, plasma, and other biological fluids, supporting hormone research, clinical trials, and pharmaceutical studies.
Q: What is the detection range and sensitivity of this ELISA kit?
A: The kit offers a detection range of 10 pg/mL to 3000 pg/mL, with a sensitivity of less than 5 pg/mL, making it suitable for low-concentration analysis.
Q: When should samples be stored and processed for optimal results?
A: Samples should be collected and stored at 2-8C before testing. Process the samples as soon as possible for reliable and consistent results.
Q: Where can this kit be used and what species does it react with?
A: Validated for human samples, it also has potential cross-reactivity with other mammals, making it suitable for a variety of research settings globally.
Q: What are the main benefits of using the E2 ELISA Kit?
A: Users benefit from its high accuracy (recovery rate 90-110%), terrific precision (intra-assay CV <10%), and comprehensive kit contents, enabling streamlined and reproducible assays.
Q: How does the competitive inhibition assay principle work in this kit?
A: The assay operates on the enzyme-linked immunosorbent principle with competitive binding, where sample estradiol competes with labeled estradiol for antibody sites, allowing quantitative measurement based on absorbance.