This ELISA kit can be applied to the in vitro quantitative determination of Human 11-DH-TXB2 concentrations in serum, plasma and other biological fluids.
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Human 11-DH-TXB2. During the reaction, Human 11-DH-TXB2 in the sample or standard competes with a fixed amount of Human 11-DH-TXB2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human 11-DH-TXB2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm Â± 2 nm. The concentration of Human 11-DH-TXB2 in the samples is then determined by comparing the OD of the samples to the standard curve.
|Detection Range||15.63â1000 pg/mL|
|Sample Type||Serum, plasma and other biological fluids|
This kit recognizes Human 11-DH-TXB2 in samples. No significant cross-reactivity or interference was observed.
The recovery of Human 11-DH-TXB2 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
|Sample Type||Range (%)||Average Recovery (%)|
|EDTA plasma (n=5)||93-108||99|
|Cell culture media (n=5)||89-102||96|
Samples were spiked with high concentrations of Human ACTH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
|Serum (n=5)||EDTA plasma (n=5)||Cell culture media (n=5)|
The unopened kit can be stored at 4â for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.
|Micro ELISA Plate(Dismountable)||8 wells Ã12 strips||-20â, 6 months|
|Reference Standard||2 vials|
|Concentrated Biotinylated Detection Ab (100Ã)||1 vial, 120 Î¼L|
|Concentrated HRP Conjugate (100Ã)||1 vial, 120 Î¼L||-20â(shading light), 6 months|
|Reference Standard & Sample Diluent||1 vial, 20 mL||4â, 6 months|
|Biotinylated Detection Ab Diluent||1 vial, 14 mL|
|HRP Conjugate Diluent||1 vial, 14 mL|
|Concentrated Wash Buffer (25Ã)||1 vial, 30 mL|
|Substrate Reagent||1 vial, 10 mL||4â(shading light)|
|Stop Solution||1 vial, 10 mL||4â|
|Plate Sealer||5 pieces|
|Product Description||1 copy|
|Certificate of Analysis||1 copy|