FAQ's of Human AT III(Antithrombin III) ELISA Kit:
Q: How do I use the Human AT III ELISA Kit for best results?
A: Carefully follow the protocol provided in the kit. Add your samples (serum, plasma, or other biological fluids), apply standards, and run the assay on a microplate reader at 450 nm. The colorimetric results are interpreted via the standard curve for accurate quantification.
Q: What is the sample volume required and what types can be tested?
A: Typically, 50-100 L of sample per well is needed. The kit is validated for serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluids.
Q: When and why should I choose this kit over others?
A: Choose this kit for high sensitivity (as low as 2.31 ng/mL), wide detection range, excellent intra-/inter-assay precision, and rapid results (within 3-3.5 hours). It's especially beneficial for research requiring high specificity and robust quantification of AT III.
Q: Where can I purchase the Human AT III ELISA Kit and are samples available?
A: This kit is available through our authorized distributors, suppliers, and traders across India. Samples can be requested for evaluation, typically offered at a minimal charge.
Q: What packaging and storage conditions are recommended?
A: Each kit comes in sturdy packaging to maintain reagent integrity. Store all components at 2-8C and avoid freeze-thaw cycles to ensure consistent performance and a shelf life of 6-12 months.
Q: How does the detection process work in this kit?
A: The kit uses a sandwich ELISA format where the antigen (AT III) binds specifically to pre-coated antibodies. The addition of the HRP-conjugate substrate leads to a colorimetric change, which is then quantified using a microplate reader.